Set1 is required for meiotic S-phase onset, double-strand break formation and middle gene expression

Julie Sollier, Waka Lin, Christine Soustelle, Karsten Suhre, Alain Nicolas, Vincent Géli, Christophe De La Roche Saint-André

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Abstract

The Set1 protein of Saccharomyces cerevisiae is a histone methyltransferase (HMTase) acting on lysine 4 of histone H3. Inactivation of the SET1 gene in a diploid leads to a sporulation defect. We have studied various processes that take place during meiotic differentiation in set1Δ diploid cells. The absence of Set1 leads to a delay of meiotic S-phase onset, which reflects a defect in DNA replication initiation. The timely induction of meiotic DNA replication does not require the Set1 HMTase activity, but depends on the SET domain. In addition, set1Δ displays a severe impairment of the DNA double-strand break formation, which is not only the consequence of the replication delay. Transcriptional profiling experiments show that the induction of middle meiotic genes, but not of early meiotic genes, is affected by the loss of Set1. In contrast to meiotic replication, the transcriptional induction of the middle meiotic genes appears to depend on the methylation of H3-K4. Our results unveil multiple roles of Set1 in meiotic differentiation and distinguish between HMTase-dependent and -independent Set1 functions.

Original languageEnglish
Pages (from-to)1957-1967
Number of pages11
JournalEMBO Journal
Volume23
Issue number9
DOIs
Publication statusPublished - 5 May 2004

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Keywords

  • Double-strand breaks
  • Gene expression
  • Meiosis
  • Replication
  • Set1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Sollier, J., Lin, W., Soustelle, C., Suhre, K., Nicolas, A., Géli, V., & De La Roche Saint-André, C. (2004). Set1 is required for meiotic S-phase onset, double-strand break formation and middle gene expression. EMBO Journal, 23(9), 1957-1967. https://doi.org/10.1038/sj.emboj.7600204