Sequence-Specific Detection of MicroRNAs by Signal-Amplifying Ribozymes

Jörg S. Hartig, Imke Grüne, Hani Najafi, Michael Famulok

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The rational and straightforward design of hairpin ribozymes that can be sequence-specifically induced by external oligonucleotides is described. Due to intrinsic signal amplification, their sensitivity is at least an order of magnitude increased compared to standard molecular beacons. We applied this system to the detection of microRNAs, a recently discovered class of small endogenous RNA molecules that are involved in gene regulation. We show that the cognate microRNA can reliably and sensitively be detected at low concentrations in a mix of other microRNA sequences. These probes may be useful in applications that require direct detection of minute amounts of small DNAs or RNAs.

Original languageEnglish
Pages (from-to)722-723
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number3
DOIs
Publication statusPublished - 28 Jan 2004
Externally publishedYes

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Catalytic RNA
RNA
MicroRNAs
Oligonucleotides
Gene expression
Amplification
DNA
Molecules
Genes

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Sequence-Specific Detection of MicroRNAs by Signal-Amplifying Ribozymes. / Hartig, Jörg S.; Grüne, Imke; Najafi, Hani; Famulok, Michael.

In: Journal of the American Chemical Society, Vol. 126, No. 3, 28.01.2004, p. 722-723.

Research output: Contribution to journalArticle

Hartig, Jörg S. ; Grüne, Imke ; Najafi, Hani ; Famulok, Michael. / Sequence-Specific Detection of MicroRNAs by Signal-Amplifying Ribozymes. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 3. pp. 722-723.
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