Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin

Arnone Nithichanon, Darawan Rinchai, Alessandro Gori, Patricia Lassaux, Claudio Peri, Oscar Conchillio-Solé, Mario Ferrer-Navarro, Louise J. Gourlay, Marco Nardini, Jordi Vila, Xavier Daura, Giorgio Colombo, Martino Bolognesi, Ganjana Lertmemonkolchai

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequencebased epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.

Original languageEnglish
Article numberA016
Pages (from-to)1-20
Number of pages20
JournalPLoS Neglected Tropical Diseases
Volume9
Issue number7
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Burkholderia pseudomallei
Flagellin
Epitopes
Melioidosis
Vaccines
Peptides
Subunit Vaccines
T-Lymphocyte Epitopes
Antibodies
Gram-Negative Bacteria
Human Activities
Computer Simulation
Communicable Diseases
Virulence
Blood Cells
Neutrophils
B-Lymphocytes
Immunoglobulin G
Cytokines
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Nithichanon, A., Rinchai, D., Gori, A., Lassaux, P., Peri, C., Conchillio-Solé, O., ... Lertmemonkolchai, G. (2015). Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin. PLoS Neglected Tropical Diseases, 9(7), 1-20. [A016]. https://doi.org/10.1371/journal.pntd.0003917

Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin. / Nithichanon, Arnone; Rinchai, Darawan; Gori, Alessandro; Lassaux, Patricia; Peri, Claudio; Conchillio-Solé, Oscar; Ferrer-Navarro, Mario; Gourlay, Louise J.; Nardini, Marco; Vila, Jordi; Daura, Xavier; Colombo, Giorgio; Bolognesi, Martino; Lertmemonkolchai, Ganjana.

In: PLoS Neglected Tropical Diseases, Vol. 9, No. 7, A016, 01.01.2015, p. 1-20.

Research output: Contribution to journalArticle

Nithichanon, A, Rinchai, D, Gori, A, Lassaux, P, Peri, C, Conchillio-Solé, O, Ferrer-Navarro, M, Gourlay, LJ, Nardini, M, Vila, J, Daura, X, Colombo, G, Bolognesi, M & Lertmemonkolchai, G 2015, 'Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin', PLoS Neglected Tropical Diseases, vol. 9, no. 7, A016, pp. 1-20. https://doi.org/10.1371/journal.pntd.0003917
Nithichanon, Arnone ; Rinchai, Darawan ; Gori, Alessandro ; Lassaux, Patricia ; Peri, Claudio ; Conchillio-Solé, Oscar ; Ferrer-Navarro, Mario ; Gourlay, Louise J. ; Nardini, Marco ; Vila, Jordi ; Daura, Xavier ; Colombo, Giorgio ; Bolognesi, Martino ; Lertmemonkolchai, Ganjana. / Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin. In: PLoS Neglected Tropical Diseases. 2015 ; Vol. 9, No. 7. pp. 1-20.
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