Sensitization of voltage activated calcium channel currents for capsaicin in nociceptive neurons by tumor-necrosis-factor-α

T. Hagenacker, J. C. Czeschik, M. Schäfers, Dietrich Busselberg

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

It is known that application of tumor-necrosis-factor-α (TNF-α) sensitizes neuronal calcium channels for heat stimuli in rat models of neuropathic pain. This study examines whether TNF-α modulates the capsaicin-induced effects after transient receptor potential vanilloid (TRPV)-1 receptor activation on voltage activated calcium channel currents (ICa(V)). TRPV-1 receptors are activated by heat and play an important role in the pathogenesis of thermal hyperalgesia in neuropathic pain syndromes, while voltage activated channels are essential for transmission of neuronal signals. Eliciting ICa(V) in DRG neurons of rats by a depolarization from the resting potential to 0 mV, TNF-α (100 ng/ml) reduces ICa(V) by 16.9 ± 2.2%, while capsaicin (0.1 μM) decreases currents by 27 ± 4.3%. Pre-application of TNF-α (100 ng/ml) for 24 h results in a sensitization of ICa(V) to capsaicin (0.1 μM) with a reduction of 42.8 ± 4.4% mediated by TRPV-1. While L-type (36.6 ± 5.2%) and P/Q-type currents (35.6 ± 4.1%) are also sensitized by TRPV-1 activation, N-type channel currents are most sensitive (74.5 ± 7.3%). The capsaicin-induced shift towards the hyperpolarizing voltage range does not occur when TNF-α is applied. Summarizing, TNF-α sensitizes nociceptive neurons for capsaicin.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalBrain Research Bulletin
Volume81
Issue number1
DOIs
Publication statusPublished - 15 Jan 2010
Externally publishedYes

Fingerprint

Nociceptors
Capsaicin
Calcium Channels
Tumor Necrosis Factor-alpha
Neuralgia
Hot Temperature
Diagnosis-Related Groups
Hyperalgesia
Membrane Potentials
Neurons
vanilloid receptor subtype 1

Keywords

  • Capsaicin
  • Electrophysiology
  • Neuropathic pain
  • TRPV-1
  • Tumor-necrosis-factor-α
  • Voltage activated calcium channels

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sensitization of voltage activated calcium channel currents for capsaicin in nociceptive neurons by tumor-necrosis-factor-α. / Hagenacker, T.; Czeschik, J. C.; Schäfers, M.; Busselberg, Dietrich.

In: Brain Research Bulletin, Vol. 81, No. 1, 15.01.2010, p. 157-163.

Research output: Contribution to journalArticle

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