Second-End Capture in DNA Double-Strand Break Repair Promoted by Brh2 Protein of Ustilago maydis

Nayef Mazloum, William K. Holloman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Brh2 plays a central role in the homologous recombination system of Ustilago maydis, mediating delivery of Rad51 to single-stranded DNA. Here we report that Brh2 can pair the displaced strand of a D loop with a complementary single-stranded DNA to form a duplexed, or double, D loop. The reaction emulates the second-end capture step envisioned in models of DNA double-strand break repair. This second-end capture reaction promoted by Brh2 proceeds efficiently when performed in the presence of Rad51 under conditions that block annealing by Rad52, or when the second single-stranded DNA substrate is replaced by double-stranded DNA. In a coupled reaction that requires extension of the D loop more than 200 nt by DNA synthesis in order to reveal a complementary region, Brh2 was also able to promote second-end capture and thus model a synthesis-dependent strand-annealing mechanism.

Original languageEnglish
Pages (from-to)160-170
Number of pages11
JournalMolecular Cell
Volume33
Issue number2
DOIs
Publication statusPublished - 30 Jan 2009
Externally publishedYes

Fingerprint

Ustilago
Double-Stranded DNA Breaks
Single-Stranded DNA
Proteins
Homologous Recombination
DNA
Complementary DNA

Keywords

  • DNA
  • MICROBIO

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Second-End Capture in DNA Double-Strand Break Repair Promoted by Brh2 Protein of Ustilago maydis. / Mazloum, Nayef; Holloman, William K.

In: Molecular Cell, Vol. 33, No. 2, 30.01.2009, p. 160-170.

Research output: Contribution to journalArticle

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