SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

Jennifer Pasquier, Nadine Abu-Kaoud, Houari Abdesselem, Aisha Madani, Jessica Hoarau, Hamda Al Thawadi, Fabien Vidal, Bettina Couderc, Gilles Favre, Arash Rafii Tabrizi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

Original languageEnglish
Article number569
JournalBMC Cancer
Volume15
Issue number1
DOIs
Publication statusPublished - 1 Aug 2015

Fingerprint

Chemokine CXCL12
Stromal Cells
Cell Communication
Breast Neoplasms
Cell Adhesion
Neoplasms
CXCR4 Receptors
Blocking Antibodies
Monoclonal Antibodies
Neoplasm Metastasis

Keywords

  • Breast cancer
  • Metastasis
  • SDF-1alpha
  • Stromal cells
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

Cite this

SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction. / Pasquier, Jennifer; Abu-Kaoud, Nadine; Abdesselem, Houari; Madani, Aisha; Hoarau, Jessica; Thawadi, Hamda Al; Vidal, Fabien; Couderc, Bettina; Favre, Gilles; Tabrizi, Arash Rafii.

In: BMC Cancer, Vol. 15, No. 1, 569, 01.08.2015.

Research output: Contribution to journalArticle

@article{f2ca2887569c4ed7b78ff8a667e666bd,
title = "SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction",
abstract = "Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.",
keywords = "Breast cancer, Metastasis, SDF-1alpha, Stromal cells, Tumor microenvironment",
author = "Jennifer Pasquier and Nadine Abu-Kaoud and Houari Abdesselem and Aisha Madani and Jessica Hoarau and Thawadi, {Hamda Al} and Fabien Vidal and Bettina Couderc and Gilles Favre and Tabrizi, {Arash Rafii}",
year = "2015",
month = "8",
day = "1",
doi = "10.1186/s12885-015-1556-7",
language = "English",
volume = "15",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

AU - Pasquier, Jennifer

AU - Abu-Kaoud, Nadine

AU - Abdesselem, Houari

AU - Madani, Aisha

AU - Hoarau, Jessica

AU - Thawadi, Hamda Al

AU - Vidal, Fabien

AU - Couderc, Bettina

AU - Favre, Gilles

AU - Tabrizi, Arash Rafii

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

AB - Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

KW - Breast cancer

KW - Metastasis

KW - SDF-1alpha

KW - Stromal cells

KW - Tumor microenvironment

UR - http://www.scopus.com/inward/record.url?scp=84938055758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938055758&partnerID=8YFLogxK

U2 - 10.1186/s12885-015-1556-7

DO - 10.1186/s12885-015-1556-7

M3 - Article

VL - 15

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

IS - 1

M1 - 569

ER -