Salivary testosterone measurement in women with and without polycystic ovary syndrome

Thozhukat Sathyapalan, Ahmed Al-Qaissi, Eric S. Kilpatrick, Soha Dargham, Joanne Adaway, Brian Keevil, Stephen Atkin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Clinical and/or biochemical hyperandrogenism is one of the diagnostic criteria for PCOS. An evaluation of the role of salivary testosterone (salT) and androstenedione (salA) for the diagnosis of PCOS was undertaken in a cross sectional study involving 65 women without PCOS and 110 women with PCOS fulfilling all 3 diagnostic Rotterdam criteria. Serum and salivary androgen measurements were determined by LC-MS/MS. salT and salA were significantly elevated in PCOS compared to controls (P < 001). No androgen marker was more predictive than another using ROC curves, but multiple logistic regression suggested salT was more predictive than free androgen index (FAI) (p < 0.01). The combination of salT or FAI identified 100% of PCOS women. PCOS women with both biochemical and clinical hyperandrogenism as opposed to clinical hyperandrogenism alone showed a metabolic phenotype (p < 0.05) and insulin resistance (p < 0.001). PCOS patients with an isolated elevated FAI showed increased insulin resistance compared to those with an isolated salT (P < 0.05). salT appeared to be at least as predictive as FAI for the diagnosis of the classical PCOS phenotype, and the combination of salT or FAI identified 100% of PCOS patients. This suggests that salT measurement by LC-MS/MS holds the promise of complementing existing laboratory tests as a means of assessing hyperandrogenemia.

Original languageEnglish
Article number3589
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Polycystic Ovary Syndrome
Testosterone
Androgens
Hyperandrogenism
Androstenedione
Insulin Resistance
Phenotype
ROC Curve
Salts
Cross-Sectional Studies
Logistic Models
Serum

ASJC Scopus subject areas

  • General

Cite this

Salivary testosterone measurement in women with and without polycystic ovary syndrome. / Sathyapalan, Thozhukat; Al-Qaissi, Ahmed; Kilpatrick, Eric S.; Dargham, Soha; Adaway, Joanne; Keevil, Brian; Atkin, Stephen.

In: Scientific Reports, Vol. 7, No. 1, 3589, 01.12.2017.

Research output: Contribution to journalArticle

Sathyapalan, Thozhukat ; Al-Qaissi, Ahmed ; Kilpatrick, Eric S. ; Dargham, Soha ; Adaway, Joanne ; Keevil, Brian ; Atkin, Stephen. / Salivary testosterone measurement in women with and without polycystic ovary syndrome. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{791cedffe3e842aaa940fbc32cad0499,
title = "Salivary testosterone measurement in women with and without polycystic ovary syndrome",
abstract = "Clinical and/or biochemical hyperandrogenism is one of the diagnostic criteria for PCOS. An evaluation of the role of salivary testosterone (salT) and androstenedione (salA) for the diagnosis of PCOS was undertaken in a cross sectional study involving 65 women without PCOS and 110 women with PCOS fulfilling all 3 diagnostic Rotterdam criteria. Serum and salivary androgen measurements were determined by LC-MS/MS. salT and salA were significantly elevated in PCOS compared to controls (P < 001). No androgen marker was more predictive than another using ROC curves, but multiple logistic regression suggested salT was more predictive than free androgen index (FAI) (p < 0.01). The combination of salT or FAI identified 100{\%} of PCOS women. PCOS women with both biochemical and clinical hyperandrogenism as opposed to clinical hyperandrogenism alone showed a metabolic phenotype (p < 0.05) and insulin resistance (p < 0.001). PCOS patients with an isolated elevated FAI showed increased insulin resistance compared to those with an isolated salT (P < 0.05). salT appeared to be at least as predictive as FAI for the diagnosis of the classical PCOS phenotype, and the combination of salT or FAI identified 100{\%} of PCOS patients. This suggests that salT measurement by LC-MS/MS holds the promise of complementing existing laboratory tests as a means of assessing hyperandrogenemia.",
author = "Thozhukat Sathyapalan and Ahmed Al-Qaissi and Kilpatrick, {Eric S.} and Soha Dargham and Joanne Adaway and Brian Keevil and Stephen Atkin",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-03945-w",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Salivary testosterone measurement in women with and without polycystic ovary syndrome

AU - Sathyapalan, Thozhukat

AU - Al-Qaissi, Ahmed

AU - Kilpatrick, Eric S.

AU - Dargham, Soha

AU - Adaway, Joanne

AU - Keevil, Brian

AU - Atkin, Stephen

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Clinical and/or biochemical hyperandrogenism is one of the diagnostic criteria for PCOS. An evaluation of the role of salivary testosterone (salT) and androstenedione (salA) for the diagnosis of PCOS was undertaken in a cross sectional study involving 65 women without PCOS and 110 women with PCOS fulfilling all 3 diagnostic Rotterdam criteria. Serum and salivary androgen measurements were determined by LC-MS/MS. salT and salA were significantly elevated in PCOS compared to controls (P < 001). No androgen marker was more predictive than another using ROC curves, but multiple logistic regression suggested salT was more predictive than free androgen index (FAI) (p < 0.01). The combination of salT or FAI identified 100% of PCOS women. PCOS women with both biochemical and clinical hyperandrogenism as opposed to clinical hyperandrogenism alone showed a metabolic phenotype (p < 0.05) and insulin resistance (p < 0.001). PCOS patients with an isolated elevated FAI showed increased insulin resistance compared to those with an isolated salT (P < 0.05). salT appeared to be at least as predictive as FAI for the diagnosis of the classical PCOS phenotype, and the combination of salT or FAI identified 100% of PCOS patients. This suggests that salT measurement by LC-MS/MS holds the promise of complementing existing laboratory tests as a means of assessing hyperandrogenemia.

AB - Clinical and/or biochemical hyperandrogenism is one of the diagnostic criteria for PCOS. An evaluation of the role of salivary testosterone (salT) and androstenedione (salA) for the diagnosis of PCOS was undertaken in a cross sectional study involving 65 women without PCOS and 110 women with PCOS fulfilling all 3 diagnostic Rotterdam criteria. Serum and salivary androgen measurements were determined by LC-MS/MS. salT and salA were significantly elevated in PCOS compared to controls (P < 001). No androgen marker was more predictive than another using ROC curves, but multiple logistic regression suggested salT was more predictive than free androgen index (FAI) (p < 0.01). The combination of salT or FAI identified 100% of PCOS women. PCOS women with both biochemical and clinical hyperandrogenism as opposed to clinical hyperandrogenism alone showed a metabolic phenotype (p < 0.05) and insulin resistance (p < 0.001). PCOS patients with an isolated elevated FAI showed increased insulin resistance compared to those with an isolated salT (P < 0.05). salT appeared to be at least as predictive as FAI for the diagnosis of the classical PCOS phenotype, and the combination of salT or FAI identified 100% of PCOS patients. This suggests that salT measurement by LC-MS/MS holds the promise of complementing existing laboratory tests as a means of assessing hyperandrogenemia.

UR - http://www.scopus.com/inward/record.url?scp=85020857352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020857352&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-03945-w

DO - 10.1038/s41598-017-03945-w

M3 - Article

AN - SCOPUS:85020857352

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3589

ER -