RSC remodeling of oligo-nucleosomes: An atomic force microscopy study

Fabien Montel, Martin Castelnovo, Hervé Menoni, Dimitar Angelov, Stefan Dimitrov, Cendrine Faivre-Moskalenko

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The 'remodels structure of chromatin' (RSC) complex is an essential chromatin remodeling factor that is required for the control of several processes including transcription, repair and replication. The ability of RSC to relocate centrally positioned mononucleosomes at the end of nucleosomal DNA is firmly established, but the data on RSC action on oligo-nucleosomal templates remains still scarce. By using atomic force microscopy (AFM) imaging, we have quantitatively studied the RSC-induced mobilization of positioned di-and trinucleosomes as well as the directionality of mobilization on mononucleosomal template labeled at one end with streptavidin. AFM imaging showed only a limited set of distinct configurational states for the remodeling products. No stepwise or preferred directionality of the nucleosome motion was observed. Analysis of the corresponding reaction pathways allows deciphering the mechanistic features of RSC-induced nucleosome relocation. The final outcome of RSC remodeling of oligosome templates is the packing of the nucleosomes at the edge of the template, providing large stretches of DNA depleted of nucleosomes. This feature of RSC may be used by the cell to overcome the barrier imposed by the presence of nucleosomes.

Original languageEnglish
Pages (from-to)2571-2579
Number of pages9
JournalNucleic Acids Research
Volume39
Issue number7
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

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Chromatin Assembly and Disassembly
Nucleosomes
Atomic Force Microscopy
Chromatin
Streptavidin
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Montel, F., Castelnovo, M., Menoni, H., Angelov, D., Dimitrov, S., & Faivre-Moskalenko, C. (2011). RSC remodeling of oligo-nucleosomes: An atomic force microscopy study. Nucleic Acids Research, 39(7), 2571-2579. https://doi.org/10.1093/nar/gkq1254

RSC remodeling of oligo-nucleosomes : An atomic force microscopy study. / Montel, Fabien; Castelnovo, Martin; Menoni, Hervé; Angelov, Dimitar; Dimitrov, Stefan; Faivre-Moskalenko, Cendrine.

In: Nucleic Acids Research, Vol. 39, No. 7, 04.2011, p. 2571-2579.

Research output: Contribution to journalArticle

Montel, F, Castelnovo, M, Menoni, H, Angelov, D, Dimitrov, S & Faivre-Moskalenko, C 2011, 'RSC remodeling of oligo-nucleosomes: An atomic force microscopy study', Nucleic Acids Research, vol. 39, no. 7, pp. 2571-2579. https://doi.org/10.1093/nar/gkq1254
Montel F, Castelnovo M, Menoni H, Angelov D, Dimitrov S, Faivre-Moskalenko C. RSC remodeling of oligo-nucleosomes: An atomic force microscopy study. Nucleic Acids Research. 2011 Apr;39(7):2571-2579. https://doi.org/10.1093/nar/gkq1254
Montel, Fabien ; Castelnovo, Martin ; Menoni, Hervé ; Angelov, Dimitar ; Dimitrov, Stefan ; Faivre-Moskalenko, Cendrine. / RSC remodeling of oligo-nucleosomes : An atomic force microscopy study. In: Nucleic Acids Research. 2011 ; Vol. 39, No. 7. pp. 2571-2579.
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