Role of the STIM1 C-terminal domain in STIM1 clustering

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Store-operated Ca2+ entry (SOCE) represents a ubiquitous Ca 2+ influx pathway activated by the filling state of intracellular Ca2+ stores. SOCE is mediated by coupling of STIM1, the endoplasmic reticulum Ca2+ sensor, to the Orai1 channel. SOCE inactivates during meiosis, partly because of the inability of STIM1 to cluster in response to store depletion. STIM1 has several functional domains, including the Orai1 interaction domain (STIM1 Orai Activating Region (SOAR) or CRAC Activation Domain (CAD)) and STIM1 homomerization domain. When Ca2+ stores are full, these domains are inactive to prevent constitutive Ca2+ entry. Here we show, using the Xenopus oocyte as an expression system, that the C-terminal 200 residues of STIM1 are important to maintain STIM1 in an inactive state when Ca2+ stores are full, through predicted intramolecular shielding of the active STIM1 domains (SOAR/CAD and STIM1 homomerization domain). Interestingly, our data argue that the C-terminal 200 residues accomplish this through a steric hindrance mechanism because they can be substituted by GFP or mCherry while maintaining all aspects of STIM1 function. We further show that STIM1 clustering inhibition during meiosis is independent of the C-terminal 200 residues.

Original languageEnglish
Pages (from-to)8375-8384
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number10
DOIs
Publication statusPublished - 11 Mar 2011

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Meiosis
Cluster Analysis
Chemical activation
Xenopus
Endoplasmic Reticulum
Shielding
Oocytes
Sensors

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Role of the STIM1 C-terminal domain in STIM1 clustering. / Yu, Fang; Sun, Lu; Courjaret, Raphael Jean; Machaca, Khaled.

In: Journal of Biological Chemistry, Vol. 286, No. 10, 11.03.2011, p. 8375-8384.

Research output: Contribution to journalArticle

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