Role of survivin phosphorylation by Aurora B in mitosis

Marlène Delacour-Larose, My Nhung Hoang Thi, Stefan Dimitrov, Annie Molla

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The chromosomal protein passenger complex, a key mitotic regulator, consists of at least four proteins, INCENP, Aurora B, Survivin and Borealin. Survivin, in contrast to the other members of the chromosomal protein passenger complex (CPC), is mobile at metaphase. This protein is also phosphorylated by Aurora B at Threonine 117. In this work we have studied the role of the phosphorylation of Survivin in mitosis by using non phosphorylable T117A and phosphomimic T117E silent resistant Survivin mutants, inducible cell lines expressing these mutants and a combination of siRNA, time-lapse microscopy and FRAP analysis. Time lapse microscopy and FRAP analysis show that Survivin T117A mutant is very stably associated with centromeres and its expression induces a prometaphasic arrest in endogenous survivin depleted cells. In addition, Survivin T117A was unable to rescue the phenotypes of the endogenous survivin depleted cells. Expressed in these cells, the phosphomimic Survivin T117E mutant exhibits a very weak interaction with the centromeres and behaves as a dominant negative mutant inducing severe mitotic defects. Our data suggest that the Aurora B generated phosphorylation/dephosphorylation cycle of Survivin is required for proper proceeding of mitosis.

Original languageEnglish
Pages (from-to)1878-1885
Number of pages8
JournalCell Cycle
Volume6
Issue number15
Publication statusPublished - 1 Aug 2007
Externally publishedYes

Fingerprint

Mitosis
Phosphorylation
Centromere
Microscopy
Proteins
Threonine
Metaphase
Small Interfering RNA
Phenotype
Cell Line

Keywords

  • Aurora B kinase
  • Mitosis
  • Passenger protein complex
  • Survivin mutants
  • Survivin phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Delacour-Larose, M., Thi, M. N. H., Dimitrov, S., & Molla, A. (2007). Role of survivin phosphorylation by Aurora B in mitosis. Cell Cycle, 6(15), 1878-1885.

Role of survivin phosphorylation by Aurora B in mitosis. / Delacour-Larose, Marlène; Thi, My Nhung Hoang; Dimitrov, Stefan; Molla, Annie.

In: Cell Cycle, Vol. 6, No. 15, 01.08.2007, p. 1878-1885.

Research output: Contribution to journalArticle

Delacour-Larose, M, Thi, MNH, Dimitrov, S & Molla, A 2007, 'Role of survivin phosphorylation by Aurora B in mitosis', Cell Cycle, vol. 6, no. 15, pp. 1878-1885.
Delacour-Larose M, Thi MNH, Dimitrov S, Molla A. Role of survivin phosphorylation by Aurora B in mitosis. Cell Cycle. 2007 Aug 1;6(15):1878-1885.
Delacour-Larose, Marlène ; Thi, My Nhung Hoang ; Dimitrov, Stefan ; Molla, Annie. / Role of survivin phosphorylation by Aurora B in mitosis. In: Cell Cycle. 2007 ; Vol. 6, No. 15. pp. 1878-1885.
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