Risk of Escherichia coli O157

H7, non-O157 Shiga toxin-producing Escherichia coli, and Campylobacter spp. in food animals and their products in Qatar

Hussni O. Mohammed, Korana Stipetic, Ahmed Salem, Patrick Mcdonough, Yung Fu Chang, Ali Sultan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Escherichia coli O157:H7, non-O157 E. Coli, and Campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. The associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. Animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bacterial enrichment and real-time PCR tests without culture confirmation. Data on putative risk factors were also collected and analyzed. E. Coli O157:H7 was detected by PCR at higher levels in sheep and camel feces than in cattle feces (odds ratios [OR], 6.8 and 21.1, respectively). Although the genes indicating E. Coli O157:H7 were detected at a relatively higher rate (4.3%) in fecal samples from dairy cattle, they were less common in milk and udder swabs from the same animals (1 and 2%, respectively). Among the food adulterants, E. Coli O103 was more common in cattle fecal samples, whereas O26 was more common in sheep feces and O45 in camel feces compared with cattle (OR, 2.6 and 3.1, respectively). The occurrence of E. Coli in the targeted populations differed by the type of sample and season of the year. Campylobacter jejuni and Campylobacter coli were more common in sheep and camel feces than in cattle feces. Most of the survey and surveillance of E. Coli focused on serogroup O157 as a potential foodborne hazard; however, based on the PCR results, non-O157 Shiga toxin-producing E. Coli serotypes appeared to be more common, and efforts should be made to include them in food safety programs.

Original languageEnglish
Pages (from-to)1812-1818
Number of pages7
JournalJournal of Food Protection
Volume78
Issue number10
DOIs
Publication statusPublished - 1 Oct 2015

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Qatar
Shiga-Toxigenic Escherichia coli
Shiga toxin-producing Escherichia coli
Campylobacter
Escherichia coli O157
food animals
Feces
Camelus
feces
camels
Food
Escherichia coli
Sheep
cattle
sheep
odds ratio
food safety
serotypes
Odds Ratio
Campylobacter coli

ASJC Scopus subject areas

  • Microbiology
  • Food Science

Cite this

Risk of Escherichia coli O157 : H7, non-O157 Shiga toxin-producing Escherichia coli, and Campylobacter spp. in food animals and their products in Qatar. / Mohammed, Hussni O.; Stipetic, Korana; Salem, Ahmed; Mcdonough, Patrick; Chang, Yung Fu; Sultan, Ali.

In: Journal of Food Protection, Vol. 78, No. 10, 01.10.2015, p. 1812-1818.

Research output: Contribution to journalArticle

Mohammed, Hussni O. ; Stipetic, Korana ; Salem, Ahmed ; Mcdonough, Patrick ; Chang, Yung Fu ; Sultan, Ali. / Risk of Escherichia coli O157 : H7, non-O157 Shiga toxin-producing Escherichia coli, and Campylobacter spp. in food animals and their products in Qatar. In: Journal of Food Protection. 2015 ; Vol. 78, No. 10. pp. 1812-1818.
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abstract = "Escherichia coli O157:H7, non-O157 E. Coli, and Campylobacter spp. are among the top-ranked pathogens that threaten the safety of food supply systems around the world. The associated risks and predisposing factors were investigated in a dynamic animal population using a repeat-cross-sectional study design. Animal and environmental samples were collected from dairy and camel farms, chicken processing plants, and abattoirs and analyzed for the presence of these pathogens using a combination of bacterial enrichment and real-time PCR tests without culture confirmation. Data on putative risk factors were also collected and analyzed. E. Coli O157:H7 was detected by PCR at higher levels in sheep and camel feces than in cattle feces (odds ratios [OR], 6.8 and 21.1, respectively). Although the genes indicating E. Coli O157:H7 were detected at a relatively higher rate (4.3{\%}) in fecal samples from dairy cattle, they were less common in milk and udder swabs from the same animals (1 and 2{\%}, respectively). Among the food adulterants, E. Coli O103 was more common in cattle fecal samples, whereas O26 was more common in sheep feces and O45 in camel feces compared with cattle (OR, 2.6 and 3.1, respectively). The occurrence of E. Coli in the targeted populations differed by the type of sample and season of the year. Campylobacter jejuni and Campylobacter coli were more common in sheep and camel feces than in cattle feces. Most of the survey and surveillance of E. Coli focused on serogroup O157 as a potential foodborne hazard; however, based on the PCR results, non-O157 Shiga toxin-producing E. Coli serotypes appeared to be more common, and efforts should be made to include them in food safety programs.",
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