Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS

Maurizio Ceppi, Giovanna Clavarino, Evelina Gatti, Enrico K. Schmidt, Aude De Gassart, Derek Blankenship, Gerald Ogola, Jacques Banchereau, Damien J. Chaussabel, Philippe Pierre

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background. Dendritic cells (DCs) are the sentinels of the mammalian immune system, characterized by a complex maturation process driven by pathogen detection. Although multiple studies have described the analysis of activated DCs by transcriptional profiling, recent findings indicate that mRNAs are also regulated at the translational level. A systematic analysis of the mRNAs being translationally regulated at various stages of DC activation was performed using translational profiling, which combines sucrose gradient fractionation of polysomal-bound mRNAs with DNA microarray analysis. Results. Total and polysomal-bound mRNA populations purified from immature, 4 h and 16 h LPS-stimulated human monocyte-derived DCs were analyzed on Affymetrix microarrays U133 2.0. A group of 375 transcripts was identified as translationally regulated during DC-activation. In addition to several biochemical pathways related to immunity, the most statistically relevant biological function identified among the translationally regulated mRNAs was protein biosynthesis itself. We singled-out a cluster of 11 large ribosome proteins mRNAs, which are disengaged from polysomes at late time of maturation, suggesting the existence of a negative feedback loop regulating translation in DCs and linking ribosomal proteins to immuno-modulatory function. Conclusion. Our observations highlight the importance of translation regulation during the immune response, and may favor the identification of novel protein networks relevant for immunity. Our study also provides information on the potential absence of correlation between gene expression and protein production for specific mRNA molecules present in DCs.

Original languageEnglish
Article number5
JournalImmunome Research
Volume5
Issue number1
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Dendritic Cells
Ribosomal Proteins
Messenger RNA
Activation
Chemical activation
Proteins
Protein
Immunity
Microarrays
Profiling
Monocytes
Microarray Analysis
DNA Microarray
Polyribosomes
Negative Feedback
Immune system
Immune Response
Biosynthesis
Immune System
Sugar (sucrose)

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Computer Science Applications
  • Computational Theory and Mathematics
  • Applied Mathematics

Cite this

Ceppi, M., Clavarino, G., Gatti, E., Schmidt, E. K., De Gassart, A., Blankenship, D., ... Pierre, P. (2009). Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS. Immunome Research, 5(1), [5]. https://doi.org/10.1186/1745-7580-5-5

Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS. / Ceppi, Maurizio; Clavarino, Giovanna; Gatti, Evelina; Schmidt, Enrico K.; De Gassart, Aude; Blankenship, Derek; Ogola, Gerald; Banchereau, Jacques; Chaussabel, Damien J.; Pierre, Philippe.

In: Immunome Research, Vol. 5, No. 1, 5, 2009.

Research output: Contribution to journalArticle

Ceppi, M, Clavarino, G, Gatti, E, Schmidt, EK, De Gassart, A, Blankenship, D, Ogola, G, Banchereau, J, Chaussabel, DJ & Pierre, P 2009, 'Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS', Immunome Research, vol. 5, no. 1, 5. https://doi.org/10.1186/1745-7580-5-5
Ceppi, Maurizio ; Clavarino, Giovanna ; Gatti, Evelina ; Schmidt, Enrico K. ; De Gassart, Aude ; Blankenship, Derek ; Ogola, Gerald ; Banchereau, Jacques ; Chaussabel, Damien J. ; Pierre, Philippe. / Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS. In: Immunome Research. 2009 ; Vol. 5, No. 1.
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