Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals

Dario Bonanomi, Onanong Chivatakarn, Ge Bai, Houari Abdesselem, Karen Lettieri, Till Marquardt, Brian A. Pierchala, Samuel L. Pfaff

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Growing axons encounter multiple guidance cues, but it is unclear how separate signals are resolved and integrated into coherent instructions for growth cone navigation. We report that glycosylphosphatidylinositol (GPI)-anchored ephrin-As function as "reverse" signaling receptors for motor axons when contacted by transmembrane EphAs present in the dorsal limb. Ephrin-A receptors are thought to depend on transmembrane coreceptors for transmitting signals intracellularly. We show that the receptor tyrosine kinase Ret is required for motor axon attraction mediated by ephrin-A reverse signaling. Ret also mediates GPI-anchored GFRα1 signaling in response to GDNF, a diffusible chemoattractant in the limb, indicating that Ret is a multifunctional coreceptor for guidance molecules. Axons respond synergistically to coactivation by GDNF and EphA ligands, and these cooperative interactions are gated by GFRα1 levels. Our studies uncover a hierarchical GPI-receptor signaling network that is constructed from combinatorial components and integrated through Ret using ligand coincidence detection.

Original languageEnglish
Pages (from-to)568-582
Number of pages15
JournalCell
Volume148
Issue number3
DOIs
Publication statusPublished - 3 Feb 2012
Externally publishedYes

Fingerprint

Glycosylphosphatidylinositols
Axons
Ephrins
Glial Cell Line-Derived Neurotrophic Factor
Extremities
Eph Family Receptors
Ligands
Growth Cones
Chemotactic Factors
Receptor Protein-Tyrosine Kinases
Cues
Cones
Navigation
Molecules
Axon Guidance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bonanomi, D., Chivatakarn, O., Bai, G., Abdesselem, H., Lettieri, K., Marquardt, T., ... Pfaff, S. L. (2012). Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals. Cell, 148(3), 568-582. https://doi.org/10.1016/j.cell.2012.01.024

Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals. / Bonanomi, Dario; Chivatakarn, Onanong; Bai, Ge; Abdesselem, Houari; Lettieri, Karen; Marquardt, Till; Pierchala, Brian A.; Pfaff, Samuel L.

In: Cell, Vol. 148, No. 3, 03.02.2012, p. 568-582.

Research output: Contribution to journalArticle

Bonanomi, D, Chivatakarn, O, Bai, G, Abdesselem, H, Lettieri, K, Marquardt, T, Pierchala, BA & Pfaff, SL 2012, 'Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals', Cell, vol. 148, no. 3, pp. 568-582. https://doi.org/10.1016/j.cell.2012.01.024
Bonanomi, Dario ; Chivatakarn, Onanong ; Bai, Ge ; Abdesselem, Houari ; Lettieri, Karen ; Marquardt, Till ; Pierchala, Brian A. ; Pfaff, Samuel L. / Ret is a multifunctional coreceptor that integrates diffusible- and contact-axon guidance signals. In: Cell. 2012 ; Vol. 148, No. 3. pp. 568-582.
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