Responses of the human airway epithelium transcriptome to in vivo injury

Adriana Heguy, Ben Gary Harvey, Philip L. Leopold, Igor Dolgalev, Tina Raman, Ronald Crystal

Research output: Contribution to journalArticle

36 Citations (Scopus)


To identify genes participating in human airway epithelial repair, we used bronchoscopy and brushing to denude the airway epithelium of healthy individuals, sequentially sampled the same region 7 and 14 days later, and assessed gene expression by Affymetrix microarrays with TaqMan RT-PCR confirmation. Histologically, the injured area was completely covered by a partially redifferentiated epithelial layer after 7 days; by 14 days the airway epithelium was very similar to the uninjured state. At day 7 compared with resting epithelium, there were substantial differences in gene expression pattern, with a distinctive airway epithelial "repair transcriptome" of actively proliferating cells in the process of redifferentiation. The repair transcriptome at 7 days was dominated by cell cycle, signal transduction, metabolism and transport, and transcription genes. Interestingly, the majority of differentially expressed cell cycle genes belonged to the G2 and M phases, suggesting that the proliferating cells were relatively synchronized 1 wk following injury. At 14 days postinjury, the expression profile was similar to that of resting airway epithelium. These observations provide a baseline of the functional gene categories participating in the process of normal human airway epithelial repair that can be used in future studies of injury and repair in airway epithelial diseases.

Original languageEnglish
Pages (from-to)139-148
Number of pages10
JournalPhysiological Genomics
Issue number2
Publication statusPublished - 24 Apr 2007
Externally publishedYes



  • Cell cycle
  • Gene expression
  • Lung
  • Microarray
  • Repair

ASJC Scopus subject areas

  • Physiology
  • Genetics

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