Resolving the three-dimensional histology of the heart

Matthew Gibb, Rebecca A B Burton, Christian Bollensdorff, Carlos Afonso, Tahir Mansoori, Ulrich Schotten, Davig J. Gavaghan, Blanca Rodriguez, Jurgen E. Schneider, Peter Kohl, Vicente Grau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

Cardiac histo-anatomical structure is a key determinant in all aspects of cardiac function. While some characteristics of micro- and macrostructure can be quantified using non-invasive imaging methods, histology is still the modality that provides the best combination of resolution and identification of cellular/sub-cellular substrate identities. The main limitation of histology is that it does not provide inherently consistent three-dimensional (3D) volume representations. This paper presents methods developed within our group to reconstruct 3D histological datasets. It includes the use of high-resolution MRI and block-face images to provide supporting volumetric datasets to guide spatial reintegration of 2D histological section data, and presents recent developments in sample preparation, data acquisition, and image processing.

Original languageEnglish
Title of host publicationLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Pages2-16
Number of pages15
Volume7605 LNBI
DOIs
Publication statusPublished - 30 Oct 2012
Externally publishedYes
Event10th International Conference on Computational Methods in Systems Biology, CMSB 2012 - London, United Kingdom
Duration: 3 Oct 20125 Oct 2012

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume7605 LNBI
ISSN (Print)03029743
ISSN (Electronic)16113349

Other

Other10th International Conference on Computational Methods in Systems Biology, CMSB 2012
CountryUnited Kingdom
CityLondon
Period3/10/125/10/12

Fingerprint

Histology
Cardiac
Three-dimensional
Data Acquisition
Magnetic resonance imaging
Modality
Microstructure
Image Processing
Data acquisition
Preparation
Determinant
Image processing
High Resolution
Substrate
Imaging
Face
Imaging techniques
Substrates
Heart

Keywords

  • Cardiac imaging
  • cardiac microstructure
  • histology
  • three-dimensional reconstruction

ASJC Scopus subject areas

  • Computer Science(all)
  • Theoretical Computer Science

Cite this

Gibb, M., Burton, R. A. B., Bollensdorff, C., Afonso, C., Mansoori, T., Schotten, U., ... Grau, V. (2012). Resolving the three-dimensional histology of the heart. In Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) (Vol. 7605 LNBI, pp. 2-16). (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 7605 LNBI). https://doi.org/10.1007/978-3-642-33636-2_2

Resolving the three-dimensional histology of the heart. / Gibb, Matthew; Burton, Rebecca A B; Bollensdorff, Christian; Afonso, Carlos; Mansoori, Tahir; Schotten, Ulrich; Gavaghan, Davig J.; Rodriguez, Blanca; Schneider, Jurgen E.; Kohl, Peter; Grau, Vicente.

Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). Vol. 7605 LNBI 2012. p. 2-16 (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 7605 LNBI).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gibb, M, Burton, RAB, Bollensdorff, C, Afonso, C, Mansoori, T, Schotten, U, Gavaghan, DJ, Rodriguez, B, Schneider, JE, Kohl, P & Grau, V 2012, Resolving the three-dimensional histology of the heart. in Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). vol. 7605 LNBI, Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), vol. 7605 LNBI, pp. 2-16, 10th International Conference on Computational Methods in Systems Biology, CMSB 2012, London, United Kingdom, 3/10/12. https://doi.org/10.1007/978-3-642-33636-2_2
Gibb M, Burton RAB, Bollensdorff C, Afonso C, Mansoori T, Schotten U et al. Resolving the three-dimensional histology of the heart. In Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). Vol. 7605 LNBI. 2012. p. 2-16. (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)). https://doi.org/10.1007/978-3-642-33636-2_2
Gibb, Matthew ; Burton, Rebecca A B ; Bollensdorff, Christian ; Afonso, Carlos ; Mansoori, Tahir ; Schotten, Ulrich ; Gavaghan, Davig J. ; Rodriguez, Blanca ; Schneider, Jurgen E. ; Kohl, Peter ; Grau, Vicente. / Resolving the three-dimensional histology of the heart. Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics). Vol. 7605 LNBI 2012. pp. 2-16 (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)).
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