Remosomes

RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer

Manu Shubhdarshan Shukla, Sajad Hussain Syed, Fabien Montel, Cendrine Faivre-Moskalenko, Jan Bednar, Andrew Travers, Dimitar Angelov, Stefan Dimitrov

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Chromatin remodelers are sophisticated nano-machines that are able to alter histone-DNA interactions and to mobilize nucleosomes. Neither the mechanism of their action nor the conformation of the remodeled nucleosomes are, however, yet well understood. We have studied the mechanism of Remodels Structure of Chromatin (RSC)-nucleosome mobilization by using high-resolution microscopy and biochemical techniques. Atomic force microscopy and electron cryomicroscopy (EC-M) analyses show that two types of products are generated during the RSC remodeling: (i) stable non-mobilized particles, termed remosomes that contain about 180 bp of DNA associated with the histone octamer and, (ii) mobilized particles located at the end of DNA. EC-M reveals that individual remosomes exhibit a distinct, variable, highly-irregular DNA trajectory. The use of the unique "one pot assays" for studying the accessibility of nucleosomal DNA towards restriction enzymes, DNase I footprinting and ExoIII mapping demonstrate that the histone-DNA interactions within the remosomes are strongly perturbed, particularly in the vicinity of the nucleosome dyad. The data suggest a two-step mechanism of RSC-nucleosome remodeling consisting of an initial formation of a remosome followed by mobilization. In agreement with this model, we show experimentally that the remosomes are intermediate products generated during the first step of the remodeling reaction that are further efficiently mobilized by RSC.

Original languageEnglish
Pages (from-to)1936-1941
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number5
DOIs
Publication statusPublished - 2 Feb 2010
Externally publishedYes

Fingerprint

Nucleosomes
Histones
Chromatin
DNA
Cryoelectron Microscopy
Chromatin Assembly and Disassembly
Atomic Force Microscopy
Deoxyribonuclease I
DNA Restriction Enzymes
Microscopy

Keywords

  • Intermediate
  • Mechanism
  • Nucleosome
  • Remodeling
  • Sliding

ASJC Scopus subject areas

  • General

Cite this

Remosomes : RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer. / Shukla, Manu Shubhdarshan; Syed, Sajad Hussain; Montel, Fabien; Faivre-Moskalenko, Cendrine; Bednar, Jan; Travers, Andrew; Angelov, Dimitar; Dimitrov, Stefan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 5, 02.02.2010, p. 1936-1941.

Research output: Contribution to journalArticle

Shukla, Manu Shubhdarshan ; Syed, Sajad Hussain ; Montel, Fabien ; Faivre-Moskalenko, Cendrine ; Bednar, Jan ; Travers, Andrew ; Angelov, Dimitar ; Dimitrov, Stefan. / Remosomes : RSC generated non-mobilized particles with approximately 180 bp DNA loosely associated with the histone octamer. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 5. pp. 1936-1941.
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