Remodeling sperm chromatin in Xenopus laevis egg extracts

The role of core histone phosphorylation and linker histone B4 in chromatin assembly

Stefan Dimitrov, Mary C. Dasso, Alan P. Wolffe

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

We find that the remodeling of the condensed Xenopus laevis sperm nucleus into the paternal pronucleus in egg extracts is associated with phosphorylation of the core histones H2A, H2A.X and H4, and uptake of a linker histone B4 and a HMG 2 protein. Histone B4 is required for the assembly of chromatosome structures in the pronucleus. However neither B4 nor core histone phosphorylation are required for the assembly of spaced nucleosomal arrays. We suggest that the spacing of nucleosomal arrays is determined by interaction between adjacent histone octamers under physiological assembly conditions.

Original languageEnglish
Pages (from-to)591-601
Number of pages11
JournalJournal of Cell Biology
Volume126
Issue number3
DOIs
Publication statusPublished - Aug 1994
Externally publishedYes

Fingerprint

Chromatin Assembly and Disassembly
Xenopus laevis
Histones
Ovum
Spermatozoa
Phosphorylation
HMGB2 Protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Remodeling sperm chromatin in Xenopus laevis egg extracts : The role of core histone phosphorylation and linker histone B4 in chromatin assembly. / Dimitrov, Stefan; Dasso, Mary C.; Wolffe, Alan P.

In: Journal of Cell Biology, Vol. 126, No. 3, 08.1994, p. 591-601.

Research output: Contribution to journalArticle

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