Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1

Christoph Peter, Michaela Waibel, Hildegard Keppeler, Rainer Lehmann, Guowang Xu, Anna Halama, Jerzy Adamski, Klaus Schulze-Osthoff, Sebastian Wesselborg, Kirsten Lauber

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Efficient engulfment of apoptotic cells is essential in multi-cellular organisms in order to prevent inflammatory responses. Apoptotic cells secure this process by releasing 'find-me' signals for the attraction of phagocytes. A major 'find-me' signal liberated from apoptotic cells is lysophosphatidylcholine (LPC). So far, however, the mechanisms underlying LPC release are poorly understood. In this study, we demonstrate that pharmacological inhibition and RNAi-mediated knock-down of the lipid transporter ABCA1 in apoptotic cells completely abolished phagocyte attraction. Moreover, ectopic expression of ABCA1 significantly enhanced monocyte migration to supernatants of apoptotic cells. Hence, ABCA1 represents a novel regulator of LPC release during apoptosis.

Original languageEnglish
Pages (from-to)568-573
Number of pages6
JournalAutoimmunity
Volume45
Issue number8
DOIs
Publication statusPublished - 1 Dec 2012
Externally publishedYes

Fingerprint

Lysophospholipids
ATP-Binding Cassette Transporters
Lysophosphatidylcholines
Apoptosis
Phagocytes
RNA Interference
Monocytes
Pharmacology
Lipids

Keywords

  • 'find-me' signal
  • Apoptosis
  • ATP-binding cassette transporter
  • Chemotaxis
  • Lysophosphatidylcholine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1. / Peter, Christoph; Waibel, Michaela; Keppeler, Hildegard; Lehmann, Rainer; Xu, Guowang; Halama, Anna; Adamski, Jerzy; Schulze-Osthoff, Klaus; Wesselborg, Sebastian; Lauber, Kirsten.

In: Autoimmunity, Vol. 45, No. 8, 01.12.2012, p. 568-573.

Research output: Contribution to journalArticle

Peter, C, Waibel, M, Keppeler, H, Lehmann, R, Xu, G, Halama, A, Adamski, J, Schulze-Osthoff, K, Wesselborg, S & Lauber, K 2012, 'Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1', Autoimmunity, vol. 45, no. 8, pp. 568-573. https://doi.org/10.3109/08916934.2012.719947
Peter, Christoph ; Waibel, Michaela ; Keppeler, Hildegard ; Lehmann, Rainer ; Xu, Guowang ; Halama, Anna ; Adamski, Jerzy ; Schulze-Osthoff, Klaus ; Wesselborg, Sebastian ; Lauber, Kirsten. / Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1. In: Autoimmunity. 2012 ; Vol. 45, No. 8. pp. 568-573.
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