Regulation of transforming growth factor-β1 (TGF-β1) expression with a novel TGF-β1 complementary DNA

Ashwani Khanna, Baogui Li, Peixin Li, Manikkam Suthanthiran

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We describe here a novel TGF-β1 complementary DNA (antisense oligomer) that is specific for TGF-β1 genomic DNA. The TGF-β1 antisense oligomer, complementary to the nucleotides flanking the first transcription start site of the human TGF-β1 gene and phosphorothioate modified, was efficacious in: a) constraining TGF-β1 promoter activity; b) reducing TGF-β1 secretion; and c) preventing TGF-β1 dependent inhibition of DNA synthesis in TGF-β sensitive A-549 human adenocarcinoma cells. The biologic activities of the TGF-β1 antisense oligomer were sequence specific since neither the TGF-β1 sense oligomer nor the TGF-β1 missense oligomer prevented TGF-β1 expression. Our findings, in addition to demonstrating the efficacy and specificity of the TGF-β1 antisense oligomer, suggest that the oligomer might be of value for the treatment of diseases in which TGF-β1 overexpression might play a pathogenetic role (e.g., diabetic renal disease, AIDS).

Original languageEnglish
Pages (from-to)1061-1066
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume204
Issue number3
DOIs
Publication statusPublished - 15 Nov 1994
Externally publishedYes

Fingerprint

Transforming Growth Factors
Complementary DNA
Oligomers
Transcription Initiation Site
DNA
Acquired Immunodeficiency Syndrome
Adenocarcinoma
Nucleotides
Genes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Regulation of transforming growth factor-β1 (TGF-β1) expression with a novel TGF-β1 complementary DNA. / Khanna, Ashwani; Li, Baogui; Li, Peixin; Suthanthiran, Manikkam.

In: Biochemical and Biophysical Research Communications, Vol. 204, No. 3, 15.11.1994, p. 1061-1066.

Research output: Contribution to journalArticle

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