Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation

Gabriela Galicia-Vázquez, Sergio Di Marco, Xian J. Lian, Jennifer F. Ma, Imed Gallouzi, Jerry Pelletier

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Gene expression during muscle cell differentiation is tightly regulated at multiple levels, including translation initiation. The PI3K/mTOR signalling pathway exerts control over protein synthesis by regulating assembly of eukaryotic initiation factor (eIF) 4F, a heterotrimeric complex that stimulates recruitment of ribosomes to mRNA templates. One of the subunits of eIF4F, eIF4A, supplies essential helicase function during this phase of translation. The presence of two cellular eIF4A isoforms, eIF4AI and eIF4AII, has long thought to impart equivalent functions to eIF4F. However, recent experiments have alluded to distinct activities between them. Herein, we characterize distinct regulatory mechanisms between the eIF4A isoforms during muscle cell differentiation. We find that eIF4AI levels decrease during differentiation whereas eIF4AII levels increase during myofiber formation in a MyoD-dependent manner. This study characterizes a previously undefined mechanism for eIF4AII regulation in differentiation and highlights functional differences between eIF4AI and eIF4AII. Finally, RNAi-mediated alterations in eIF4AI and eIF4AII levels indicate that the myogenic process can tolerate short term reductions in eIF4AI or eIF4AII levels, but not both.

Original languageEnglish
Article numbere87237
JournalPLoS One
Volume9
Issue number1
DOIs
Publication statusPublished - 23 Jan 2014
Externally publishedYes

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Eukaryotic Initiation Factors
cell differentiation
myocytes
Muscle Cells
translation (genetics)
Eukaryotic Initiation Factor-4F
Muscle
Cell Differentiation
Protein Isoforms
Cells
mice
phosphatidylinositol 3-kinase
RNA Interference
ribosomes
Phosphatidylinositol 3-Kinases
Ribosomes
Gene expression
protein synthesis
Gene Expression
gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. / Galicia-Vázquez, Gabriela; Di Marco, Sergio; Lian, Xian J.; Ma, Jennifer F.; Gallouzi, Imed; Pelletier, Jerry.

In: PLoS One, Vol. 9, No. 1, e87237, 23.01.2014.

Research output: Contribution to journalArticle

Galicia-Vázquez, Gabriela ; Di Marco, Sergio ; Lian, Xian J. ; Ma, Jennifer F. ; Gallouzi, Imed ; Pelletier, Jerry. / Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. In: PLoS One. 2014 ; Vol. 9, No. 1.
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