Reductive genome evolution from the mother of Rickettsia.

Guillaume Blanc, Hiroyuki Ogata, Catherine Robert, Stéphane Audic, Karsten Suhre, Guy Vestris, Jean Michel Claverie, Didier Raoult

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

The Rickettsia genus is a group of obligate intracellular alpha-proteobacteria representing a paradigm of reductive evolution. Here, we investigate the evolutionary processes that shaped the genomes of the genus. The reconstruction of ancestral genomes indicates that their last common ancestor contained more genes, but already possessed most traits associated with cellular parasitism. The differences in gene repertoires across modern Rickettsia are mainly the result of differential gene losses from the ancestor. We demonstrate using computer simulation that the propensity of loss was variable across genes during this process. We also analyzed the ratio of nonsynonymous to synonymous changes (Ka/Ks) calculated as an average over large sets of genes to assay the strength of selection acting on the genomes of Rickettsia, Anaplasmataceae, and free-living gamma-proteobacteria. As a general trend, Ka/Ks were found to decrease with increasing divergence between genomes. The high Ka/Ks for closely related genomes are probably due to a lag in the removal of slightly deleterious nonsynonymous mutations by natural selection. Interestingly, we also observed a decrease of the rate of gene loss with increasing divergence, suggesting a similar lag in the removal of slightly deleterious pseudogene alleles. For larger divergence (Ks > 0.2), Ka/Ks converge toward similar values indicating that the levels of selection are roughly equivalent between intracellular alpha-proteobacteria and their free-living relatives. This contrasts with the view that obligate endocellular microorganisms tend to evolve faster as a consequence of reduced effectiveness of selection, and suggests a major role of enhanced background mutation rates on the fast protein divergence in the obligate intracellular alpha-proteobacteria.

Original languageEnglish
JournalPLoS Genetics
Volume3
Issue number1
DOIs
Publication statusPublished - 19 Jan 2007
Externally publishedYes

Fingerprint

Rickettsia
genome
Genome
Alphaproteobacteria
gene
alpha-Proteobacteria
divergence
Genes
genes
Anaplasmataceae
mutation
ancestry
Gammaproteobacteria
Pseudogenes
pseudogenes
Genetic Selection
gamma-Proteobacteria
common ancestry
Mutation Rate
parasitism

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Blanc, G., Ogata, H., Robert, C., Audic, S., Suhre, K., Vestris, G., ... Raoult, D. (2007). Reductive genome evolution from the mother of Rickettsia. PLoS Genetics, 3(1). https://doi.org/10.1371/journal.pgen.0030014

Reductive genome evolution from the mother of Rickettsia. / Blanc, Guillaume; Ogata, Hiroyuki; Robert, Catherine; Audic, Stéphane; Suhre, Karsten; Vestris, Guy; Claverie, Jean Michel; Raoult, Didier.

In: PLoS Genetics, Vol. 3, No. 1, 19.01.2007.

Research output: Contribution to journalArticle

Blanc, G, Ogata, H, Robert, C, Audic, S, Suhre, K, Vestris, G, Claverie, JM & Raoult, D 2007, 'Reductive genome evolution from the mother of Rickettsia.', PLoS Genetics, vol. 3, no. 1. https://doi.org/10.1371/journal.pgen.0030014
Blanc, Guillaume ; Ogata, Hiroyuki ; Robert, Catherine ; Audic, Stéphane ; Suhre, Karsten ; Vestris, Guy ; Claverie, Jean Michel ; Raoult, Didier. / Reductive genome evolution from the mother of Rickettsia. In: PLoS Genetics. 2007 ; Vol. 3, No. 1.
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