Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity

Garrett Heinrich, Harrison T. Muturi, Khadijeh Rezaei, Qusai Y. Al-Share, Anthony M. DeAngelis, Thomas A. Bowman, Hilda E. Ghadieh, Simona Ghanem, Deqiang Zhang, Robert S. Garofalo, Lei Yin, Sonia M. Najjar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Impairment of insulin clearance is being increasingly recognized as a critical step in the development of insulin resistance and metabolic disease. The carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) promotes insulin clearance. Null deletion or liver-specific inactivation of Ceacam1 in mice causes a defect in insulin clearance, insulin resistance, steatohepatitis, and visceral obesity. Immunohistological analysis revealed reduction of hepatic CEACAM1 in obese subjects with fatty liver disease. Thus, we aimed to determine whether this occurs at the hepatocyte level in response to systemic extrahepatic factors and whether this holds across species. Northern and Western blot analyses demonstrate that CEACAM1 mRNA and protein levels are reduced in liver tissues of obese individuals compared to their lean age-matched counterparts. Furthermore, Western analysis reveals a comparable reduction of CEACAM1 protein in primary hepatocytes derived from the same obese subjects. Similar to humans, Ceacam1 mRNA level, assessed by quantitative RT-PCR analysis, is significantly reduced in the livers of obese Zucker (fa/fa, ZDF) and Koletsky (f/f) rats relative to their age-matched lean counterparts. These studies demonstrate that the reduction of hepatic CEACAM1 in obesity occurs at the level of hepatocytes and identify the reduction of hepatic CEACAM1 as a common denominator of obesity across multiple species.

Original languageEnglish
Article number54
JournalFrontiers in Endocrinology
Volume8
Issue numberMAR
DOIs
Publication statusPublished - 27 Mar 2017
Externally publishedYes

Fingerprint

Carcinoembryonic Antigen
Cell Adhesion Molecules
Obesity
Liver
Hepatocytes
Fatty Liver
Insulin
Insulin Resistance
Messenger RNA
Abdominal Obesity
Metabolic Diseases
Northern Blotting
Liver Diseases
Proteins
Western Blotting
Polymerase Chain Reaction

Keywords

  • Carcinoembryonic antigen-related cell adhesion molecule 1
  • Fatty liver disease
  • Hyperinsulinemia
  • Insulin clearance
  • Insulin resistance
  • Obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

Heinrich, G., Muturi, H. T., Rezaei, K., Al-Share, Q. Y., DeAngelis, A. M., Bowman, T. A., ... Najjar, S. M. (2017). Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity. Frontiers in Endocrinology, 8(MAR), [54]. https://doi.org/10.3389/fendo.2017.00054

Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity. / Heinrich, Garrett; Muturi, Harrison T.; Rezaei, Khadijeh; Al-Share, Qusai Y.; DeAngelis, Anthony M.; Bowman, Thomas A.; Ghadieh, Hilda E.; Ghanem, Simona; Zhang, Deqiang; Garofalo, Robert S.; Yin, Lei; Najjar, Sonia M.

In: Frontiers in Endocrinology, Vol. 8, No. MAR, 54, 27.03.2017.

Research output: Contribution to journalArticle

Heinrich, G, Muturi, HT, Rezaei, K, Al-Share, QY, DeAngelis, AM, Bowman, TA, Ghadieh, HE, Ghanem, S, Zhang, D, Garofalo, RS, Yin, L & Najjar, SM 2017, 'Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity', Frontiers in Endocrinology, vol. 8, no. MAR, 54. https://doi.org/10.3389/fendo.2017.00054
Heinrich G, Muturi HT, Rezaei K, Al-Share QY, DeAngelis AM, Bowman TA et al. Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity. Frontiers in Endocrinology. 2017 Mar 27;8(MAR). 54. https://doi.org/10.3389/fendo.2017.00054
Heinrich, Garrett ; Muturi, Harrison T. ; Rezaei, Khadijeh ; Al-Share, Qusai Y. ; DeAngelis, Anthony M. ; Bowman, Thomas A. ; Ghadieh, Hilda E. ; Ghanem, Simona ; Zhang, Deqiang ; Garofalo, Robert S. ; Yin, Lei ; Najjar, Sonia M. / Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity. In: Frontiers in Endocrinology. 2017 ; Vol. 8, No. MAR.
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