Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice

Andrea Pavirani, Tim Skem, Marianne Le Meur, Yves Lutz, Richard Lathe, Ronald Crystal, Jean Paul Fuchs, Pierre Gerlinger, Michael Courtney

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

As a new approach to produce human proteins of therapeutic interest we have derived lymphoid cell lines expressing such proteins from transgenic mice, either by generating trans-hybridomas by cell fusion in vitro or by one gene mediated immortalisation in vivo of transgenic lymphocytes. The latter procedure could be applied to different cell types for the expression of proteins that require tissue specific modifications.

Original languageEnglish
Pages (from-to)1049-1054
Number of pages6
JournalNature Biotechnology
Volume7
Issue number10
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

Fingerprint

Recombinant proteins
Recombinant Proteins
Transgenic Mice
Cells
Lymphocytes
Proteins
Cell Line
Cell Fusion
Hybridomas
Fusion reactions
Therapeutics
Genes
Tissue

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice. / Pavirani, Andrea; Skem, Tim; Meur, Marianne Le; Lutz, Yves; Lathe, Richard; Crystal, Ronald; Fuchs, Jean Paul; Gerlinger, Pierre; Courtney, Michael.

In: Nature Biotechnology, Vol. 7, No. 10, 01.01.1989, p. 1049-1054.

Research output: Contribution to journalArticle

Pavirani, A, Skem, T, Meur, ML, Lutz, Y, Lathe, R, Crystal, R, Fuchs, JP, Gerlinger, P & Courtney, M 1989, 'Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice', Nature Biotechnology, vol. 7, no. 10, pp. 1049-1054. https://doi.org/10.1038/nbt1089-1049
Pavirani, Andrea ; Skem, Tim ; Meur, Marianne Le ; Lutz, Yves ; Lathe, Richard ; Crystal, Ronald ; Fuchs, Jean Paul ; Gerlinger, Pierre ; Courtney, Michael. / Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice. In: Nature Biotechnology. 1989 ; Vol. 7, No. 10. pp. 1049-1054.
@article{6211daaffafc45419c54f9d5b44bcaae,
title = "Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice",
abstract = "As a new approach to produce human proteins of therapeutic interest we have derived lymphoid cell lines expressing such proteins from transgenic mice, either by generating trans-hybridomas by cell fusion in vitro or by one gene mediated immortalisation in vivo of transgenic lymphocytes. The latter procedure could be applied to different cell types for the expression of proteins that require tissue specific modifications.",
author = "Andrea Pavirani and Tim Skem and Meur, {Marianne Le} and Yves Lutz and Richard Lathe and Ronald Crystal and Fuchs, {Jean Paul} and Pierre Gerlinger and Michael Courtney",
year = "1989",
month = "1",
day = "1",
doi = "10.1038/nbt1089-1049",
language = "English",
volume = "7",
pages = "1049--1054",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Recombinant proteins of therapeutic interest expressed by lymphoid cell lines derived from transgenic mice

AU - Pavirani, Andrea

AU - Skem, Tim

AU - Meur, Marianne Le

AU - Lutz, Yves

AU - Lathe, Richard

AU - Crystal, Ronald

AU - Fuchs, Jean Paul

AU - Gerlinger, Pierre

AU - Courtney, Michael

PY - 1989/1/1

Y1 - 1989/1/1

N2 - As a new approach to produce human proteins of therapeutic interest we have derived lymphoid cell lines expressing such proteins from transgenic mice, either by generating trans-hybridomas by cell fusion in vitro or by one gene mediated immortalisation in vivo of transgenic lymphocytes. The latter procedure could be applied to different cell types for the expression of proteins that require tissue specific modifications.

AB - As a new approach to produce human proteins of therapeutic interest we have derived lymphoid cell lines expressing such proteins from transgenic mice, either by generating trans-hybridomas by cell fusion in vitro or by one gene mediated immortalisation in vivo of transgenic lymphocytes. The latter procedure could be applied to different cell types for the expression of proteins that require tissue specific modifications.

UR - http://www.scopus.com/inward/record.url?scp=0024448353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024448353&partnerID=8YFLogxK

U2 - 10.1038/nbt1089-1049

DO - 10.1038/nbt1089-1049

M3 - Article

VL - 7

SP - 1049

EP - 1054

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 10

ER -