Abstract
In the course of infection, human immunodeficiency virus type 1 (HIV-1) mutates, diverging into a "swarm" of viral quasispecies, and the predominance of CCR5- or CXCR4-utilizing quasispecies is strongly associated with the pattern of disease progression. Quantification of CCR5- and CXCR4-utilizing viruses in viral swarms is important in the investigation of the mechanisms of this phenomenon. Here, we report on a new real-time PCR-based methology for the evaluation of replication of individual CCR5- and CXCR4-utilizing variants. The assay is highly reproducible, with a coefficient of variation of <3%, and it accurately estimates the numbers of virus-specific RNA copies even when their difference in the mixture is 2 orders of magnitude. We demonstrate that replications of CCR5- and CXCR4-utilizing variants can be evaluated and distinguished in experimentally coinfected human lymphoid tissue. The assay we developed may facilitate study of the mechanisms of the R5-to-X4 switch in viral swarms in human tissues infected with HIV-1.
| Original language | English |
|---|---|
| Pages (from-to) | 2126-2131 |
| Number of pages | 6 |
| Journal | Journal of Clinical Microbiology |
| Volume | 41 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 May 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology (medical)
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Real-time PCR assay of individual human immunodeficiency virus type 1 variants in coinfected human lymphoid tissues. / Ito, Yoshinori; Grivel, Jean-Charles B.; Margolis, Leonid.
In: Journal of Clinical Microbiology, Vol. 41, No. 5, 01.05.2003, p. 2126-2131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real-time PCR assay of individual human immunodeficiency virus type 1 variants in coinfected human lymphoid tissues
AU - Ito, Yoshinori
AU - Grivel, Jean-Charles B.
AU - Margolis, Leonid
PY - 2003/5/1
Y1 - 2003/5/1
N2 - In the course of infection, human immunodeficiency virus type 1 (HIV-1) mutates, diverging into a "swarm" of viral quasispecies, and the predominance of CCR5- or CXCR4-utilizing quasispecies is strongly associated with the pattern of disease progression. Quantification of CCR5- and CXCR4-utilizing viruses in viral swarms is important in the investigation of the mechanisms of this phenomenon. Here, we report on a new real-time PCR-based methology for the evaluation of replication of individual CCR5- and CXCR4-utilizing variants. The assay is highly reproducible, with a coefficient of variation of <3%, and it accurately estimates the numbers of virus-specific RNA copies even when their difference in the mixture is 2 orders of magnitude. We demonstrate that replications of CCR5- and CXCR4-utilizing variants can be evaluated and distinguished in experimentally coinfected human lymphoid tissue. The assay we developed may facilitate study of the mechanisms of the R5-to-X4 switch in viral swarms in human tissues infected with HIV-1.
AB - In the course of infection, human immunodeficiency virus type 1 (HIV-1) mutates, diverging into a "swarm" of viral quasispecies, and the predominance of CCR5- or CXCR4-utilizing quasispecies is strongly associated with the pattern of disease progression. Quantification of CCR5- and CXCR4-utilizing viruses in viral swarms is important in the investigation of the mechanisms of this phenomenon. Here, we report on a new real-time PCR-based methology for the evaluation of replication of individual CCR5- and CXCR4-utilizing variants. The assay is highly reproducible, with a coefficient of variation of <3%, and it accurately estimates the numbers of virus-specific RNA copies even when their difference in the mixture is 2 orders of magnitude. We demonstrate that replications of CCR5- and CXCR4-utilizing variants can be evaluated and distinguished in experimentally coinfected human lymphoid tissue. The assay we developed may facilitate study of the mechanisms of the R5-to-X4 switch in viral swarms in human tissues infected with HIV-1.
UR - http://www.scopus.com/inward/record.url?scp=0037952605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037952605&partnerID=8YFLogxK
U2 - 10.1128/JCM.41.5.2126-2131.2003
DO - 10.1128/JCM.41.5.2126-2131.2003
M3 - Article
C2 - 12734258
AN - SCOPUS:0037952605
VL - 41
SP - 2126
EP - 2131
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 5
ER -