Reactive oxidative species enhance amyloid toxicity in APP/PS1 mouse neurons

Bin Yang, Xiaqin Sun, Hilal Lashuel, Yan Zhang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective To investigate whether intracellular amyloid β (iAβ) induces toxicity in wild type (WT) and APP/PS1 mice, a mouse model of Alzheimer's disease. Methods: Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1 mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species (ROS) were measured by MitoSOX Red mitochondrial superoxide indicator. Results: Crude, monomer and protofibril Aβ induced more toxicity in APP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. Conclusion: Oxidative stress may mediate cell death induced by iAβ in neurons.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalNeuroscience Bulletin
Volume28
Issue number3
DOIs
Publication statusPublished - 1 Jun 2012
Externally publishedYes

Fingerprint

Amyloid
Neurons
Cell Death
In Situ Nick-End Labeling
Superoxides
Alzheimer Disease
Oxidative Stress
Staining and Labeling

Keywords

  • Aggregation
  • Amyloid
  • APP/PS1
  • Reactive oxidative species
  • Toxicity

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Reactive oxidative species enhance amyloid toxicity in APP/PS1 mouse neurons. / Yang, Bin; Sun, Xiaqin; Lashuel, Hilal; Zhang, Yan.

In: Neuroscience Bulletin, Vol. 28, No. 3, 01.06.2012, p. 233-239.

Research output: Contribution to journalArticle

Yang, Bin ; Sun, Xiaqin ; Lashuel, Hilal ; Zhang, Yan. / Reactive oxidative species enhance amyloid toxicity in APP/PS1 mouse neurons. In: Neuroscience Bulletin. 2012 ; Vol. 28, No. 3. pp. 233-239.
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