Rapid induction of anti-idiotypic responses to unmodified monoclonal antibodies from syngeneic mice following primary immunization

Jean-Charles B. Grivel, Pierre Ferrier, Nathalie Renard, Gilles Jolly, Thérèse Jarry, Lee Leserman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A single foot-pad immunization in adjuvant of BALB/c mice with non-modified BALB/c monoclonal antibodies (HyHEL 5, 9 and 10) specific for hen egg lysozyme permitted isolation of anti-idiotypic monoclonal antibodies 10 days later. An evaluation of different screening tests revealed that antibodies were detected more easily by isotype-specific or direct binding assays than by cross-linking ELISA procedures. These results were confirmed by a direct cell-binding assay on B cells transgenic for one of the immunizing antibodies. The use of these cells also permitted an evaluation of the ability of these antibodies to inhibit antigen binding under conditions in which the target antibody, in its cell-surface configuration, is minimally modified by potential artifacts induced by purification or fixation to a solid support. This study demonstrates that anti-idiotypic responses to anti-protein antibodies may be rapidly generated in syngeneic animals.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalJournal of Immunological Methods
Volume158
Issue number2
DOIs
Publication statusPublished - 3 Feb 1993
Externally publishedYes

Fingerprint

Immunization
Monoclonal Antibodies
Antibodies
Anti-Idiotypic Antibodies
Artifacts
Foot
B-Lymphocytes
Enzyme-Linked Immunosorbent Assay
Antigens
Proteins

Keywords

  • Idiotypy
  • Immunotherapy
  • Lysozyme
  • Transgenic mouse

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Rapid induction of anti-idiotypic responses to unmodified monoclonal antibodies from syngeneic mice following primary immunization. / Grivel, Jean-Charles B.; Ferrier, Pierre; Renard, Nathalie; Jolly, Gilles; Jarry, Thérèse; Leserman, Lee.

In: Journal of Immunological Methods, Vol. 158, No. 2, 03.02.1993, p. 173-182.

Research output: Contribution to journalArticle

Grivel, Jean-Charles B. ; Ferrier, Pierre ; Renard, Nathalie ; Jolly, Gilles ; Jarry, Thérèse ; Leserman, Lee. / Rapid induction of anti-idiotypic responses to unmodified monoclonal antibodies from syngeneic mice following primary immunization. In: Journal of Immunological Methods. 1993 ; Vol. 158, No. 2. pp. 173-182.
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