Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: Population analysis of adenovirus capsid association with cells is predictive of adenovirus infectivity

T. Vincent, B. G. Harvey, S. M. Hogan, C. J. Bailey, Ronald Crystal, P. L. Leopold

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.

Original languageEnglish
Pages (from-to)1516-1521
Number of pages6
JournalJournal of Virology
Volume75
Issue number3
DOIs
Publication statusPublished - 5 Feb 2001
Externally publishedYes

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Capsid
Neutralizing Antibodies
Adenoviridae
Serum
Population
Viral Genes
Virus Diseases
Antiviral Agents
Gene Expression
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

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AU - Vincent, T.

AU - Harvey, B. G.

AU - Hogan, S. M.

AU - Bailey, C. J.

AU - Crystal, Ronald

AU - Leopold, P. L.

PY - 2001/2/5

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