Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: Population analysis of adenovirus capsid association with cells is predictive of adenovirus infectivity

T. Vincent, B. G. Harvey, S. M. Hogan, C. J. Bailey, Ronald Crystal, P. L. Leopold

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20 Citations (Scopus)

Abstract

Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.

Original languageEnglish
Pages (from-to)1516-1521
Number of pages6
JournalJournal of Virology
Volume75
Issue number3
DOIs
Publication statusPublished - 5 Feb 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Virology

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