Quantitative proteomics and dynamic imaging reveal that G3BP-mediated stress granule assembly is poly(ADP-ribose)-dependent following exposure to MNNG-induced DNA alkylation

Maxim Isabelle, Jean Philippe Gagné, Imed Gallouzi, Guy G. Poirier

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Poly(ADP-ribose) (pADPr) is a heterogenic molecule synthesised from NAD by poly(ADP-ribose) polymerases (PARPs). Many cellular functions from genome integrity surveillance, cell cycle progression and DNA repair to apoptosis are affected by pADPr through its network of associated proteins. Using quantitative proteomics, we established a temporal map of pADPr-associated complexes upon genotoxic stress. Results suggested a strong pADPr association to many proteins involved in stress granule formation, notably the ras-GAP SH3-binding protein G3BP, as well as in the later phases of alkylation-stress-induced responses. Further investigation with dynamic imaging clearly demonstrated a pADPr-dependent initiation of stress granule assembly originating from the nucleus. The cotransfection of G3BP with poly(ADP-ribose) glycohydrolase (PARG) indicates that pADPr is involved in modulating the nuclear translocation of G3BP. Moreover, a peptide pADPr blot assay of G3BP revealed that pADPr binds to the glycine-arginine-rich domain of G3BP. Thereafter, we established a comprehensive G3BP interactome in the presence of pADPr. Our findings establish a novel function for pADPr in the formation of G3BP-induced stress granules upon genotoxic stress.

Original languageEnglish
Pages (from-to)4555-4566
Number of pages12
JournalJournal of Cell Science
Volume125
Issue number19
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Poly Adenosine Diphosphate Ribose
Methylnitronitrosoguanidine
Alkylation
Proteomics
DNA
DNA Damage
ras GTPase-Activating Proteins
Poly(ADP-ribose) Polymerases
DNA Repair
NAD
Glycine
Arginine
Cell Cycle
Carrier Proteins
Proteins
Genome
Apoptosis

Keywords

  • G3BP
  • pADPr
  • Quantitative proteomics
  • SILAC
  • Stress granules

ASJC Scopus subject areas

  • Cell Biology

Cite this

Quantitative proteomics and dynamic imaging reveal that G3BP-mediated stress granule assembly is poly(ADP-ribose)-dependent following exposure to MNNG-induced DNA alkylation. / Isabelle, Maxim; Gagné, Jean Philippe; Gallouzi, Imed; Poirier, Guy G.

In: Journal of Cell Science, Vol. 125, No. 19, 2012, p. 4555-4566.

Research output: Contribution to journalArticle

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