Quantification of l-alanyl-l-glutamine in mammalian cell culture broth

Evaluation of different detectors

Jens O. Krömer, Stefanie Dietmair, Shana S. Jacob, Lars K. Nielsen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

l-Alanyl-l-glutamine (also known as Ala-Gln or GlutaMAX) is widely used as a stable l-glutamine source in cell culture for the production of biopharmaceuticals. System approaches for the optimization of production processes require the analysis of all major substrates and products. We have compared four alternative detection systems for l-alanyl-l-glutamine in culture broth. Matrix effects prevented the use of ultraviolet or evaporative light scattering detection. Fluorescence detection used in routine amino acid protocols is compatible with culture broth and has a broad linear dynamic range. Mass spectrometry has superior sensitivity and can be integrated into quantitative metabolomic workflows.

Original languageEnglish
Pages (from-to)129-131
Number of pages3
JournalAnalytical Biochemistry
Volume416
Issue number1
DOIs
Publication statusPublished - 1 Sep 2011
Externally publishedYes

Fingerprint

alanylglutamine
Glutamine
Cell culture
Cell Culture Techniques
Detectors
Metabolomics
Workflow
Light scattering
Mass spectrometry
Mass Spectrometry
Fluorescence
Light
Amino Acids
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Quantification of l-alanyl-l-glutamine in mammalian cell culture broth : Evaluation of different detectors. / Krömer, Jens O.; Dietmair, Stefanie; Jacob, Shana S.; Nielsen, Lars K.

In: Analytical Biochemistry, Vol. 416, No. 1, 01.09.2011, p. 129-131.

Research output: Contribution to journalArticle

Krömer, Jens O. ; Dietmair, Stefanie ; Jacob, Shana S. ; Nielsen, Lars K. / Quantification of l-alanyl-l-glutamine in mammalian cell culture broth : Evaluation of different detectors. In: Analytical Biochemistry. 2011 ; Vol. 416, No. 1. pp. 129-131.
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