Qualitative and quantitative changes in skeletal muscle mtDNA and expression of mitochondrial-encoded genes in the human aging process

Antoni Barrientos, Jordi Casademont, Francesc Cardellach, Esther Ardite, Xavier P. Estivill, Alvaro Urbano-Márquez, J. Carlos Fernández-Checa, Virginia Nunes

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

It has been widely postulated that age-dependent changes in the mitochondrial genetic system may contribute to the human aging process. We recently reported unchanged specific activities of mitochondrial respiratory chain enzymes and a decrease in oxidation capacity of different substrates with aging, due, in part, to some confounding variables such as physical activity or tobacco consumption. The present study deals with age-related changes in muscle mtDNA structure and its biogenesis in humans. We found a low prevalence of mtDNA rearrangements with aging, only detected by PCR. The mtDNA content increased significantly with age (b = 0.0115, P < 0.0001). Also, an unchanged steady-state level of mitochondrial transcripts, a reduced transcription rate (P < 0.0001), and an increase in mitochondrial membrane lipid peroxidation (P < 0.0001) were observed in aging. These data demonstrate that minor structural mtDNA changes appear during the human aging process. By contrast, alterations in mitochondrial homeostasis ultimately producing modifications in mitochondrial biogenesis rates could play a role in the process of human senescence.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalBiochemical and Molecular Medicine
Volume62
Issue number2
DOIs
Publication statusPublished - Dec 1997
Externally publishedYes

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Mitochondrial Genes
Mitochondrial DNA
Muscle
Skeletal Muscle
Genes
Aging of materials
Confounding Factors (Epidemiology)
Mitochondrial Membranes
Tobacco Use
Organelle Biogenesis
Membrane Lipids
Electron Transport
Tobacco
Lipid Peroxidation
Transcription
Homeostasis
Muscles
Polymerase Chain Reaction
Enzymes
Oxidation

Keywords

  • Aging
  • Mitochondrial transcription
  • Mitochondrial translation
  • mtDNA changes
  • OXPHOS

ASJC Scopus subject areas

  • Biochemistry

Cite this

Qualitative and quantitative changes in skeletal muscle mtDNA and expression of mitochondrial-encoded genes in the human aging process. / Barrientos, Antoni; Casademont, Jordi; Cardellach, Francesc; Ardite, Esther; Estivill, Xavier P.; Urbano-Márquez, Alvaro; Fernández-Checa, J. Carlos; Nunes, Virginia.

In: Biochemical and Molecular Medicine, Vol. 62, No. 2, 12.1997, p. 165-171.

Research output: Contribution to journalArticle

Barrientos, A, Casademont, J, Cardellach, F, Ardite, E, Estivill, XP, Urbano-Márquez, A, Fernández-Checa, JC & Nunes, V 1997, 'Qualitative and quantitative changes in skeletal muscle mtDNA and expression of mitochondrial-encoded genes in the human aging process', Biochemical and Molecular Medicine, vol. 62, no. 2, pp. 165-171. https://doi.org/10.1006/bmme.1997.2647
Barrientos, Antoni ; Casademont, Jordi ; Cardellach, Francesc ; Ardite, Esther ; Estivill, Xavier P. ; Urbano-Márquez, Alvaro ; Fernández-Checa, J. Carlos ; Nunes, Virginia. / Qualitative and quantitative changes in skeletal muscle mtDNA and expression of mitochondrial-encoded genes in the human aging process. In: Biochemical and Molecular Medicine. 1997 ; Vol. 62, No. 2. pp. 165-171.
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