Purification, functionalization, and bioconjugation of carbon nanotubes

John H.T. Luong, Keith B. Male, Khaled A. Mahmoud, Fwu Shan Sheu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)


Bioconjugation of carbon nanotubes (CNTs) with biomolecules promises exciting applications such as biosensing, nanobiocomposite formulation, design of drug vector systems, and probing protein interactions. Pristine CNTs, however, are virtually water-insoluble and difficult to evenly disperse in a liquid matrix. Therefore, it is necessary to attach molecules or functional groups to their sidewalls to enable bioconjugation. Both noncovalent and covalent procedures can be used to conjugate CNTs with a target biomolecule for a specific bioapplication. This chapter presents a few selected protocols that can be performed at any wet chemistry laboratory to purify and biofunctionalize CNTs. The preparation of CNTs modified with metallic nanoparticles, especially gold, is also described since biomolecules can bind and self-organize on the surfaces of such metal-decorated CNTs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages28
Publication statusPublished - 1 Jan 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Carbon nanotubes (CNTs)
  • Gold nanoparticles
  • Noncovalent/covalent biofunctionalization
  • Purification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Luong, J. H. T., Male, K. B., Mahmoud, K. A., & Sheu, F. S. (2011). Purification, functionalization, and bioconjugation of carbon nanotubes. In Methods in Molecular Biology (pp. 505-532). (Methods in Molecular Biology; Vol. 751). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-151-2_32