PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks

Patricia Benedit, Rosanna Paciucci, Timothy M. Thomson, Marta Valeri, Marga Nadal, Carme Càceres, Inés De Torres, Xavier P. Estivill, Juan José Lozano, Joan Morote, Jaume Reventós

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In a search for molecular markers of progression in prostate cancer by means of differential display, we have identified a new gene, which we have designated PTOV1. Semiquantitative RT - PCR has established that nine out of 11 tumors overexpress PTOV1 at levels significantly higher than benign prostatic hyperplasia or normal prostate tissue. The human PTOV1 protein consists almost entirely of two repeated blocks of homology of 151 and 147 amino acids, joined by a short linker peptide, and is encoded by a 12-exon gene localized in chromosome 19q13.3. A Drosophila melanogaster PTOV1 homolog also contains two tandemly arranged PTOV blocks. A second gene, PTOV2, was identified in humans and Drosophila, coding for proteins with a single PTOV homology block and unrelated amino- and carboxyl-terminal extensions. A 1.8-Kb PTOV1 transcript was detected abundantly in normal human brain, heart, skeletal muscle, kidney and liver, and at low levels in normal prostate. Immunocytochemical analysis and expression of chimeric GFP-PTOV1 proteins in cultured cells showed a predominantly perinuclear localization of PTOV1. In normal prostate tissue and in prostate adenomas, PTOV1 was undetectable or expressed at low levels, whereas nine out of 11 prostate adenocarcinomas showed a strong immunoreactivity, with a focal distribution in areas of carcinoma and prostatic intraepithelial neoplasia. Therefore, PTOV1 is a previously unknown gene, overexpressed in early and late stages of prostate cancer. The PTOV homology block represents a new class of conserved sequence blocks present in human, rodent and fly proteins.

Original languageEnglish
Pages (from-to)1455-1464
Number of pages10
JournalOncogene
Volume20
Issue number12
DOIs
Publication statusPublished - 22 Mar 2001
Externally publishedYes

Fingerprint

Prostate
Prostatic Neoplasms
Proteins
Genes
Prostatic Intraepithelial Neoplasia
Conserved Sequence
Prostatic Hyperplasia
Drosophila melanogaster
Diptera
Adenoma
Exons
Cultured Cells
Rodentia
Myocardium
Skeletal Muscle
Adenocarcinoma
Chromosomes
Carcinoma
Kidney
Amino Acids

Keywords

  • Androgens
  • Molecular markers
  • Prostate
  • Prostate adenocarcinoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Benedit, P., Paciucci, R., Thomson, T. M., Valeri, M., Nadal, M., Càceres, C., ... Reventós, J. (2001). PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks. Oncogene, 20(12), 1455-1464. https://doi.org/10.1038/sj.onc.1204233

PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks. / Benedit, Patricia; Paciucci, Rosanna; Thomson, Timothy M.; Valeri, Marta; Nadal, Marga; Càceres, Carme; De Torres, Inés; Estivill, Xavier P.; Lozano, Juan José; Morote, Joan; Reventós, Jaume.

In: Oncogene, Vol. 20, No. 12, 22.03.2001, p. 1455-1464.

Research output: Contribution to journalArticle

Benedit, P, Paciucci, R, Thomson, TM, Valeri, M, Nadal, M, Càceres, C, De Torres, I, Estivill, XP, Lozano, JJ, Morote, J & Reventós, J 2001, 'PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks', Oncogene, vol. 20, no. 12, pp. 1455-1464. https://doi.org/10.1038/sj.onc.1204233
Benedit P, Paciucci R, Thomson TM, Valeri M, Nadal M, Càceres C et al. PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks. Oncogene. 2001 Mar 22;20(12):1455-1464. https://doi.org/10.1038/sj.onc.1204233
Benedit, Patricia ; Paciucci, Rosanna ; Thomson, Timothy M. ; Valeri, Marta ; Nadal, Marga ; Càceres, Carme ; De Torres, Inés ; Estivill, Xavier P. ; Lozano, Juan José ; Morote, Joan ; Reventós, Jaume. / PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks. In: Oncogene. 2001 ; Vol. 20, No. 12. pp. 1455-1464.
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