Proteome profiling of virus-host interactions of wild type and attenuated measles virus strains

Anja Billing, Julia R. Kessler, Dominique Revets, Aurélie Sausy, Stephanie Schmitz, Claire Barra, Claude P. Muller

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Quantitative gel-based proteomics (2D DIGE coupled to MALDI-TOF/TOF MS) has been used to investigate the effects of different measles virus (MV) strains on the host cell proteome. A549/hSLAM cells were infected either with wild type MV strains, an attenuated vaccine or a multiple passaged Vero cell adapted strain. By including interferon beta treatment as a control it was possible to distinguish between the classical antiviral response and changes induced specifically by the different strains. Of 38 differentially expressed proteins in total (p-value ≤. 0.05, fold change ≥. 2), 18 proteins were uniquely modulated following MV infection with up to 9 proteins specific per individual strain. Interestingly, wt strains displayed distinct protein patterns particularly during the late phase of infection. Proteins were grouped into cytoskeleton, metabolism, transcription/translation, immune response and mitochondrial proteins. Bioinformatics analysis revealed mostly changes in proteins regulating cell death and apoptosis. Surprisingly, wt strains affected the cytokeratin system much stronger than the vaccine strain. To our knowledge, this is the first study on the MV-host proteome addressing interstrain differences.

Original languageEnglish
Pages (from-to)325-336
Number of pages12
JournalJournal of Proteomics
Volume108
DOIs
Publication statusPublished - 28 Aug 2014
Externally publishedYes

Fingerprint

Measles virus
Proteome
Viruses
Proteins
Two-Dimensional Difference Gel Electrophoresis
Attenuated Vaccines
Vero Cells
Interferon-beta
Mitochondrial Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Virus Diseases
Keratins
Computational Biology
Cytoskeleton
Proteomics
Cell death
Transcription
Bioinformatics
Antiviral Agents
Metabolism

Keywords

  • Interferon beta
  • Interstrain differences
  • Measles virus
  • Proteomics
  • Virus-host interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Proteome profiling of virus-host interactions of wild type and attenuated measles virus strains. / Billing, Anja; Kessler, Julia R.; Revets, Dominique; Sausy, Aurélie; Schmitz, Stephanie; Barra, Claire; Muller, Claude P.

In: Journal of Proteomics, Vol. 108, 28.08.2014, p. 325-336.

Research output: Contribution to journalArticle

Billing, Anja ; Kessler, Julia R. ; Revets, Dominique ; Sausy, Aurélie ; Schmitz, Stephanie ; Barra, Claire ; Muller, Claude P. / Proteome profiling of virus-host interactions of wild type and attenuated measles virus strains. In: Journal of Proteomics. 2014 ; Vol. 108. pp. 325-336.
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