Protein ligands mediate the CRM1-dependent export of HuR in response to heat shock

Imed Gallouzi, Christopher M. Brennan, Joan A. Steitz

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

AU-rich elements (AREs) located in the 3′ UTRs of the messenger RNAs (mRNAs) of many mammalian early response genes promote rapid mRNA turnover. HuR, an RRM-containing RNA-binding protein, specifically interacts with AREs, stabilizing these mRNAs. HuR is primarily nucleoplasmic, but shuttles between the nucleus and the cytoplasm via a domain called HNS located between RRM2 and RRM3. We recently showed that HuR interacts with two protein ligands, pp32 and APRIL, which are also shuttling proteins, but rely on NES domains recognized by CRM1 for export. Here we show that heat shock induces increased association of HuR with pp32 and APRIL through protein-protein interactions and that these ligands partially colocalize with HuR in cytoplasmic foci. HuR associations with the hnRNP complex also increase, but through RNA links. CRM1 coimmunoprecipitates with HuR only after heat shock, and nuclear export of HuR becomes sensitive to leptomycin B, an inhibitor of CRM1. Export after heat shock requires the same domains of HuR (HNS and RRM3) that are essential for binding pp32 and APRIL. In situ hybridization and coimmunoprecipitation experiments show that LMB treatment blocks both hsp70 mRNA nuclear export and its cytoplasmic interaction with HuR after heat shock. Together, our results argue that upon heat shock, HuR switches its export pathway to that of its ligands pp32 and APRIL, which involves the nuclear export factor CRM1. HuR and its ligands may be instrumental in the nuclear export of heat-shock mRNAs.

Original languageEnglish
Pages (from-to)1348-1361
Number of pages14
JournalRNA
Volume7
Issue number9
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Heat-Shock Response
Shock
Cell Nucleus Active Transport
Hot Temperature
Ligands
Messenger RNA
AU Rich Elements
Proteins
Tumor Necrosis Factor Ligand Superfamily Member 13
Heterogeneous-Nuclear Ribonucleoproteins
RNA-Binding Proteins
3' Untranslated Regions
In Situ Hybridization
Cytoplasm
RNA
Genes

Keywords

  • APRIL
  • CRM1
  • ELAV
  • hsp70 mRNA
  • HuR
  • mRNA export
  • pp32

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Protein ligands mediate the CRM1-dependent export of HuR in response to heat shock. / Gallouzi, Imed; Brennan, Christopher M.; Steitz, Joan A.

In: RNA, Vol. 7, No. 9, 2001, p. 1348-1361.

Research output: Contribution to journalArticle

Gallouzi, Imed ; Brennan, Christopher M. ; Steitz, Joan A. / Protein ligands mediate the CRM1-dependent export of HuR in response to heat shock. In: RNA. 2001 ; Vol. 7, No. 9. pp. 1348-1361.
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