Protein free microcapsules obtained from plant spores as a model for drug delivery: Ibuprofen encapsulation, release and taste masking

Alberto Diego-Taboada, Laurent Maillet, Joseph H. Banoub, Mark Lorch, Alan S. Rigby, Andrew N. Boa, Stephen Atkin, Grahame Mackenzie

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Sporopollenin exine capsules (SEC) extracted from Lycopodium clavatum spores were shown to encapsulate ibuprofen as a drug model, with 97 ± 1% efficiency as measured by recovery of the loaded drug and absence of the drug on the SEC surface by scanning electron microscopy (SEM). The encapsulated ibuprofen was shown to be unchanged from its bulk crystalline form by solid state NMR, FTIR and XRD. Essential for drug delivery applications, SEC were shown to be non-toxic to human endothelial cells and free of allergenic protein epitopes by MALDI-TOF-MS and ESI-QqToF-MS. Potential application for targeted release into the intestinal region of the gastrointestinal tract (GIT) was demonstrated by 88 ± 1% of the drug being retained in simulated gastric fluid (SGF) after 45 minutes and 85 ± 2% being released after 5 min in buffer (PBS; pH 7.4). The SEC were shown to provide significant taste masking of encapsulated ibuprofen in a double blind trial with 10 human volunteers.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalJournal of Materials Chemistry B
Volume1
Issue number5
DOIs
Publication statusPublished - 7 Feb 2013
Externally publishedYes

Fingerprint

Ibuprofen
Spores
Drug delivery
Encapsulation
Capsules
Proteins
Epitopes
Endothelial cells
Pharmaceutical Preparations
Lycopodium
Nuclear magnetic resonance
Essential Drugs
Crystalline materials
Recovery
Scanning electron microscopy
Fluids
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Fourier Transform Infrared Spectroscopy
Electron Scanning Microscopy
Gastrointestinal Tract

ASJC Scopus subject areas

  • Chemistry(all)
  • Medicine(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Protein free microcapsules obtained from plant spores as a model for drug delivery : Ibuprofen encapsulation, release and taste masking. / Diego-Taboada, Alberto; Maillet, Laurent; Banoub, Joseph H.; Lorch, Mark; Rigby, Alan S.; Boa, Andrew N.; Atkin, Stephen; Mackenzie, Grahame.

In: Journal of Materials Chemistry B, Vol. 1, No. 5, 07.02.2013, p. 707-713.

Research output: Contribution to journalArticle

Diego-Taboada, Alberto ; Maillet, Laurent ; Banoub, Joseph H. ; Lorch, Mark ; Rigby, Alan S. ; Boa, Andrew N. ; Atkin, Stephen ; Mackenzie, Grahame. / Protein free microcapsules obtained from plant spores as a model for drug delivery : Ibuprofen encapsulation, release and taste masking. In: Journal of Materials Chemistry B. 2013 ; Vol. 1, No. 5. pp. 707-713.
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