Protective effect of ursolic acid against myocardial ischemia induced by isoproterenol in rats

S. Senthil, M. Sridevi, K. V. Pugalendi

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21 Citations (Scopus)

Abstract

Sustained high levels of circulating catecholamines may induce cardiotoxicity through oxidative mechanisms. Isoproterenol is a synthetic catecholamine with increasing attention owing to this application in cardiology. The aim of the present study was to investigate the cardioprotective effects of ursolic acid against isoproterenol-induced myocardial ischemia. Normal Wistar strain rats were pretreated with UA (20, 40, and 60 mg/kg, s.c.) for 7 days and then intoxicated with isoproterenol (ISO, 85 mg/kg, s.c. for 2 consecutive days). Hearts were excised from the experimental animals and assessed for the activities of cardiac markers [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase (CPK)], the levels of lipid peroxide products [thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (HPs), and conjugated dienes (CDs)], myeloperoxidase (MPO), lipid profiles [total cholesterol (TC), free cholesterol, ester cholesterol, triglycerides (TG), free fatty acids (FFAs), and phospholipids (PLs)], and membrane-bound enzymes (total ATPase, Na +K+ATPase, Ca2+ATPase, and Mg 2ATPase). In ISO-treated group, shrinkage of cardiac markers and elevated lipid peroxidation with compromised lipid profiles in the heart where accompanied by the decreased activities of membrane-bound enzymes. The prior administration of UA significantly (p < 0.05) prevented the isoproterenol-induced alterations and restored the enzymes to near normal. These findings indicate the cardioprotective activities of UA during isoproterenol-induced myocardial ischemia.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalToxicology Mechanisms and Methods
Volume17
Issue number1
DOIs
Publication statusPublished - 1 Dec 2006

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Keywords

  • Antioxidant Enzymes
  • Cardiac Markers
  • Lipid Peroxidation
  • Myocardial Ischemia
  • Ursolic Acid

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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