Protective effect of HLA-DQB1 alleles against alloimmunization in patients with sickle cell disease

Zohreh Tatari Calderone, Heather Gordish-Dressman, Ross Fasano, Michael Riggs, Catherine Fortier, Andrew D. Campbell, Dominique Charron, Victor R. Gordeuk, Naomi L.C. Luban, Stanislav Vukmanovic, Ryad Tamouza

Research output: Contribution to journalArticle

14 Citations (Scopus)


Background: Alloimmunization or the development of alloantibodies to Red Blood Cell (RBC) antigens is considered one of the major complications after RBC transfusions in patients with sickle cell disease (SCD) and can lead to both acute and delayed hemolytic reactions. It has been suggested that polymorphisms in HLA genes, may play a role in alloimmunization. We conducted a retrospective study analyzing the influence of HLA-DRB1 and DQB1 genetic diversity on RBC-alloimmunization. Study design: Two-hundred four multi-transfused SCD patients with and without RBC-alloimmunization were typed at low/medium resolution by PCR-SSO, using IMGT-HLA Database. HLA-DRB1 and DQB1 allele frequencies were analyzed using logistic regression models, and global p-value was calculated using multiple logistic regression. Results: While only trends towards associations between HLA-DR diversity and alloimmunization were observed, analysis of HLA-DQ showed that HLA-DQ2 (p = 0.02), -DQ3 (p = 0.02) and -DQ5 (p = 0.01) alleles were significantly higher in non-alloimmunized patients, likely behaving as protective alleles. In addition, multiple logistic regression analysis showed both HLA-DQ2/6 (p = 0.01) and HLA-DQ5/5 (p = 0.03) combinations constitute additional predictor of protective status. Conclusion: Our data suggest that particular HLA-DQ alleles influence the clinical course of RBC transfusion in patients with SCD, which could pave the way towards predictive strategies.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalHuman Immunology
Issue number1
Publication statusPublished - 1 Jan 2016
Externally publishedYes



  • Alloimmunization
  • HLA-DQB1
  • HLA-DRB1
  • Red Blood Cell transfusion
  • Sickle cell disease

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Tatari Calderone, Z., Gordish-Dressman, H., Fasano, R., Riggs, M., Fortier, C., Campbell, A. D., Charron, D., Gordeuk, V. R., Luban, N. L. C., Vukmanovic, S., & Tamouza, R. (2016). Protective effect of HLA-DQB1 alleles against alloimmunization in patients with sickle cell disease. Human Immunology, 77(1), 35-40.