Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model

Nico Marr, David C. Oliver, Vincianne Laurent, Jan Poolman, Philippe Denoël, Rachel C. Fernandez

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrix™ vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis. We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections.

Original languageEnglish
Pages (from-to)4306-4311
Number of pages6
JournalVaccine
Volume26
Issue number34
DOIs
Publication statusPublished - 12 Aug 2008
Externally publishedYes

Fingerprint

Bordetella pertussis
whooping cough
Vaccines
lungs
Whooping Cough
Hemagglutinins
Lung
vaccines
toxoids
Recombinant Proteins
Acellular Vaccines
mice
Pertussis Vaccine
hemagglutinins
recombinant proteins
Diphtheria-Tetanus-acellular Pertussis Vaccines
Bordetella Infections
Antigens
Tetanus Toxoid
Diphtheria

Keywords

  • Bordetella pertussis
  • BrkA
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model. / Marr, Nico; Oliver, David C.; Laurent, Vincianne; Poolman, Jan; Denoël, Philippe; Fernandez, Rachel C.

In: Vaccine, Vol. 26, No. 34, 12.08.2008, p. 4306-4311.

Research output: Contribution to journalArticle

Marr, Nico ; Oliver, David C. ; Laurent, Vincianne ; Poolman, Jan ; Denoël, Philippe ; Fernandez, Rachel C. / Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model. In: Vaccine. 2008 ; Vol. 26, No. 34. pp. 4306-4311.
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