Programmed death ligand 1 on Burkholderia pseudomallei-infected human polymorphonuclear neutrophils impairs T cell functions

Surachat Buddhisa, Darawan Rinchai, Manabu Ato, Gregory J. Bancroft, Ganjana Lertmemongkolchai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Polymorphonuclear neutrophils (PMNs) are terminally differentiated cells that are involved in innate immune responses and form an early line of defense against pathogens. More recently, it has been shown that PMNs have immunosuppressive abilities on other immune cells. However, the effect of PMNs on T cell responses during bacterial infection remains to be determined. In this report, we examined the interaction of PMNs and T cells in response to infection with Burkholderia pseudomallei, the causative agent of human melioidosis. We observed that CD4+ T cell proliferation and IFN-γ production in response to polyclonal activators is significantly inhibited by uninfected PMNs, and to a greater extent B. pseudomallei-infected PMNs. Programmed death ligand 1 (PD-L1), a known regulator of T cell activation, is increased in mRNA expression in the blood of patients and upon infection of PMNs in vitro. The increased expression of PD-L1 was correlated with the degree of T cell inhibition in individuals with type 2 diabetes, a major risk factor of melioidosis. In vitro, addition of anti-PD-L1 Abs blocked this inhibitory activity and restored proliferation of CD4+ T cells and IFN-γ production, suggesting that PD-L1 on B. pseudomallei-infected PMNs is a regulatory molecule for the functions of T cells and may be involved in pathogenesis versus control of melioidosis.

Original languageEnglish
Pages (from-to)4413-4421
Number of pages9
JournalJournal of Immunology
Volume194
Issue number9
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

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Burkholderia pseudomallei
Neutrophils
Ligands
T-Lymphocytes
Melioidosis
Immunosuppressive Agents
Infection
Bacterial Infections
Innate Immunity
Type 2 Diabetes Mellitus
Cell Proliferation
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Programmed death ligand 1 on Burkholderia pseudomallei-infected human polymorphonuclear neutrophils impairs T cell functions. / Buddhisa, Surachat; Rinchai, Darawan; Ato, Manabu; Bancroft, Gregory J.; Lertmemongkolchai, Ganjana.

In: Journal of Immunology, Vol. 194, No. 9, 01.01.2015, p. 4413-4421.

Research output: Contribution to journalArticle

Buddhisa, Surachat ; Rinchai, Darawan ; Ato, Manabu ; Bancroft, Gregory J. ; Lertmemongkolchai, Ganjana. / Programmed death ligand 1 on Burkholderia pseudomallei-infected human polymorphonuclear neutrophils impairs T cell functions. In: Journal of Immunology. 2015 ; Vol. 194, No. 9. pp. 4413-4421.
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