Proglucagon-derived peptides in intestinal epithelial proliferation: Glucagon-like peptide-2 is a major mediator of intestinal epithelial proliferation in rats

Mohammad A. Ghatei, Robert A. Goodlad, Shahrad Taheri, Nikki Mandir, Audrey E. Brynes, Mark Jordinson, Stephen R. Bloom

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Proglucagon-derived peptides have been implicated in the control of intestinal mucosal cell division. To investigate the actions of these peptides on intestinal cell proliferation, different doses of enteroglucagon, oxyntomodulin, glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) were tested in male Wistar rats maintained on total parenteral nutrition. Crypt cell proliferation was assessed by the analysis of arrested metaphases in microdissected crypts. Enteroglucagon and oxyntomodulin had no effect on intestinal weight or cell proliferation. GLP-1 had a slight effect on stomach and small intestinal weights and on epithelial cell proliferation in the small and large intestines. GLP-2 infusion dose-dependently increased the weights of the stomach, small intestine, colon, and cecum and increased crypt cell proliferation in the small and large intestines of parenterally fed rats. In orally fed animals, GLP-2 increased intestinal weight but had little effect on proliferation. Therefore, of the proglucagon-derived peptides, GLP-2 appears to be a major mediator of intestinal epithelial proliferation.

Original languageEnglish
Article number340940
Pages (from-to)1255-1263
Number of pages9
JournalDigestive Diseases and Sciences
Volume46
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Keywords

  • Glucagon-like peptide-2
  • Intestinal epithelial proliferation
  • Proglucagon-derived peptides
  • Rat
  • Total parenteral nutrition

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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