Profilin is an effector for Daam 1 in non-canonical Wnt signaling and is required for vertebrate gastrulation

Akira Sato, Deepak K. Khadka, Wei Liu, Ritu Bharti, Loren W. Runnels, Igor B. Dawid, Raymond Habas

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Non-canonical Wnt signaling plays important roles during vertebrate embryogenesis and is required for cell motility during gastrulation. However, the molecular mechanisms of how Wnt signaling regulates modification of the actin cytoskeleton remain incompletely understood. We had previously identified the Formin homology protein Daam 1 as an important link between Dishevelled and the Rho GTPase for cytoskeletal modulation. Here, we report that Profilin 1 is an effector downstream of Daam 1 required for cytoskeletal changes. Profilin 1 interacted with the FH1 domain of Daam 1 and was localized with Daam 1 to actin stress fibers in response to Wnt signaling in mammalian cells. In addition, depletion of Profilin 1 inhibited stress fiber formation induced by non-canonical Wnt signaling. Inhibition or depletion of Profilin 1 in vivo specifically inhibited blastopore closure in Xenopus but did not affect convergent extension movements, tissue separation or neural fold closure. Our studies define a molecular pathway downstream of Daam 1 that controls Wnt-mediated cytoskeletal reorganization for a specific morphogenetic process during vertebrate gastrulation.

Original languageEnglish
Pages (from-to)4219-4231
Number of pages13
JournalDevelopment
Volume133
Issue number21
DOIs
Publication statusPublished - Nov 2006
Externally publishedYes

Fingerprint

Profilins
Gastrulation
Vertebrates
Stress Fibers
Gastrula
rho GTP-Binding Proteins
Neural Crest
Xenopus
Actin Cytoskeleton
Embryonic Development
Cell Movement
Actins
Proteins

Keywords

  • Daam1
  • Profilin
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Sato, A., Khadka, D. K., Liu, W., Bharti, R., Runnels, L. W., Dawid, I. B., & Habas, R. (2006). Profilin is an effector for Daam 1 in non-canonical Wnt signaling and is required for vertebrate gastrulation. Development, 133(21), 4219-4231. https://doi.org/10.1242/dev.02590

Profilin is an effector for Daam 1 in non-canonical Wnt signaling and is required for vertebrate gastrulation. / Sato, Akira; Khadka, Deepak K.; Liu, Wei; Bharti, Ritu; Runnels, Loren W.; Dawid, Igor B.; Habas, Raymond.

In: Development, Vol. 133, No. 21, 11.2006, p. 4219-4231.

Research output: Contribution to journalArticle

Sato, A, Khadka, DK, Liu, W, Bharti, R, Runnels, LW, Dawid, IB & Habas, R 2006, 'Profilin is an effector for Daam 1 in non-canonical Wnt signaling and is required for vertebrate gastrulation', Development, vol. 133, no. 21, pp. 4219-4231. https://doi.org/10.1242/dev.02590
Sato, Akira ; Khadka, Deepak K. ; Liu, Wei ; Bharti, Ritu ; Runnels, Loren W. ; Dawid, Igor B. ; Habas, Raymond. / Profilin is an effector for Daam 1 in non-canonical Wnt signaling and is required for vertebrate gastrulation. In: Development. 2006 ; Vol. 133, No. 21. pp. 4219-4231.
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