Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle

E. Saba, M. Origoni, G. Taccagni, D. Ferrari, C. Doglioni, A. Nava, A. Lisco, Jean-Charles B. Grivel, L. Margolis, G. Poli

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Abstract

Cervical tissue explants (CTEs) from 22 HIV-1 seronegative women were exposed to R5 HIV-1 ex vivo. Eight CTEs were productively infected in terms of HIV-1 p24 Gag release in culture supernatants, whereas 14 were not. Nonetheless, both accumulation of HIV-1 gag DNA and of p24 Gag + CD4 + T cells and macrophages occurred in both productive and, at lower levels, in nonproductive CTEs. Nonproductive CTEs differed from productive CTEs for higher secretion of C-C motif chemokine ligand 3 (CCL3) and CCL5. A post-hoc analysis revealed that all productive CTEs were established from women in their secretory phase of the menstrual cycle, whereas nonproductive CTEs were derived from women either in their secretory (28%) or proliferative (36%) menstrual cycle phases or with an atrophic endometrium (36%). Thus, our results support the epidemiological observation that sexual HIV-1 transmission from males to women as well as from women to men is more efficient during their secretory phase of the menstrual cycle.

Original languageEnglish
Pages (from-to)1081-1090
Number of pages10
JournalMucosal Immunology
Volume6
Issue number6
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

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Luteal Phase
HIV Infections
HIV-1
Chemokine CCL3
Follicular Phase
Endometrium
Macrophages
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Saba, E., Origoni, M., Taccagni, G., Ferrari, D., Doglioni, C., Nava, A., ... Poli, G. (2013). Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle. Mucosal Immunology, 6(6), 1081-1090. https://doi.org/10.1038/mi.2013.2

Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle. / Saba, E.; Origoni, M.; Taccagni, G.; Ferrari, D.; Doglioni, C.; Nava, A.; Lisco, A.; Grivel, Jean-Charles B.; Margolis, L.; Poli, G.

In: Mucosal Immunology, Vol. 6, No. 6, 11.2013, p. 1081-1090.

Research output: Contribution to journalArticle

Saba, E, Origoni, M, Taccagni, G, Ferrari, D, Doglioni, C, Nava, A, Lisco, A, Grivel, J-CB, Margolis, L & Poli, G 2013, 'Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle', Mucosal Immunology, vol. 6, no. 6, pp. 1081-1090. https://doi.org/10.1038/mi.2013.2
Saba, E. ; Origoni, M. ; Taccagni, G. ; Ferrari, D. ; Doglioni, C. ; Nava, A. ; Lisco, A. ; Grivel, Jean-Charles B. ; Margolis, L. ; Poli, G. / Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle. In: Mucosal Immunology. 2013 ; Vol. 6, No. 6. pp. 1081-1090.
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