Production of interleukin-27 by human neutrophils regulates their function during bacterial infection

Darawan Rinchai, Prasong Khaenam, Chidchamai Kewcharoenwong, Surachat Buddhisa, Rungnapa Pankla, Damien J. Chaussabel, Gregory J. Bancroft, Ganjana Lertmemongkolchai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Septicemia is the most severe form of melioidosis caused by the Gram-negative bacterium, Burkholderia pseudomallei. Here, we show that levels of IL-27p28 transcript and protein were both significantly elevated in patients with sepsis, particularly melioidosis and in patients with unfavorable disease outcome. Moreover, human monocytes/macrophages and neutrophils were the major source of IL-27 during infection. The addition of exogenous IL-27 in vitro resulted in significantly increased bacterial survival, reduced B. pseudomallei-induced oxidative burst, and enhanced IL-1β and TNF-α production by purified neutrophils from healthy subjects. Finally, blockade of endogenous IL-27 in neutrophils using soluble IL-27 receptor antagonist prior to infection led to significantly reduced survival of bacteria and decreased IL-1β, but not TNF-α production. These results indicate a potential role for IL-27 in the suppression of anti-bacterial defense mechanisms that might contribute to disease severity in sepsis. The targeting of this cytokine may be beneficial in the management of human sepsis.

Original languageEnglish
Pages (from-to)3280-3290
Number of pages11
JournalEuropean Journal of Immunology
Volume42
Issue number12
DOIs
Publication statusPublished - Dec 2012
Externally publishedYes

Fingerprint

Interleukin-27
Bacterial Infections
Neutrophils
Sepsis
Melioidosis
Burkholderia pseudomallei
Interleukin-1
Respiratory Burst
Survival
Infection
Gram-Negative Bacteria
Monocytes
Healthy Volunteers
Macrophages
human IL27 protein
Cytokines
Bacteria
Proteins

Keywords

  • Burkholderia pseudomallei
  • IL-27
  • Melioidosis
  • Neutrophil
  • Sepsis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Production of interleukin-27 by human neutrophils regulates their function during bacterial infection. / Rinchai, Darawan; Khaenam, Prasong; Kewcharoenwong, Chidchamai; Buddhisa, Surachat; Pankla, Rungnapa; Chaussabel, Damien J.; Bancroft, Gregory J.; Lertmemongkolchai, Ganjana.

In: European Journal of Immunology, Vol. 42, No. 12, 12.2012, p. 3280-3290.

Research output: Contribution to journalArticle

Rinchai, D, Khaenam, P, Kewcharoenwong, C, Buddhisa, S, Pankla, R, Chaussabel, DJ, Bancroft, GJ & Lertmemongkolchai, G 2012, 'Production of interleukin-27 by human neutrophils regulates their function during bacterial infection', European Journal of Immunology, vol. 42, no. 12, pp. 3280-3290. https://doi.org/10.1002/eji.201242526
Rinchai, Darawan ; Khaenam, Prasong ; Kewcharoenwong, Chidchamai ; Buddhisa, Surachat ; Pankla, Rungnapa ; Chaussabel, Damien J. ; Bancroft, Gregory J. ; Lertmemongkolchai, Ganjana. / Production of interleukin-27 by human neutrophils regulates their function during bacterial infection. In: European Journal of Immunology. 2012 ; Vol. 42, No. 12. pp. 3280-3290.
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