Production of interleukin-27 by human neutrophils regulates their function during bacterial infection

Darawan Rinchai, Prasong Khaenam, Chidchamai Kewcharoenwong, Surachat Buddhisa, Rungnapa Pankla, Damien J. Chaussabel, Gregory J. Bancroft, Ganjana Lertmemongkolchai

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23 Citations (Scopus)


Septicemia is the most severe form of melioidosis caused by the Gram-negative bacterium, Burkholderia pseudomallei. Here, we show that levels of IL-27p28 transcript and protein were both significantly elevated in patients with sepsis, particularly melioidosis and in patients with unfavorable disease outcome. Moreover, human monocytes/macrophages and neutrophils were the major source of IL-27 during infection. The addition of exogenous IL-27 in vitro resulted in significantly increased bacterial survival, reduced B. pseudomallei-induced oxidative burst, and enhanced IL-1β and TNF-α production by purified neutrophils from healthy subjects. Finally, blockade of endogenous IL-27 in neutrophils using soluble IL-27 receptor antagonist prior to infection led to significantly reduced survival of bacteria and decreased IL-1β, but not TNF-α production. These results indicate a potential role for IL-27 in the suppression of anti-bacterial defense mechanisms that might contribute to disease severity in sepsis. The targeting of this cytokine may be beneficial in the management of human sepsis.

Original languageEnglish
Pages (from-to)3280-3290
Number of pages11
JournalEuropean Journal of Immunology
Issue number12
Publication statusPublished - Dec 2012
Externally publishedYes



  • Burkholderia pseudomallei
  • IL-27
  • Melioidosis
  • Neutrophil
  • Sepsis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Rinchai, D., Khaenam, P., Kewcharoenwong, C., Buddhisa, S., Pankla, R., Chaussabel, D. J., Bancroft, G. J., & Lertmemongkolchai, G. (2012). Production of interleukin-27 by human neutrophils regulates their function during bacterial infection. European Journal of Immunology, 42(12), 3280-3290.