Production of fibronectin by the human alveolar macrophage

mechanism for the recruitment of fibroblasts to sites of tissue injury in interstitial lung diseases.

S. I. Rennard, G. W. Hunninghake, P. B. Bitterman, Ronald Crystal

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Because cells of the mononuclear phagocyte system are known to produce fibronectin and because alveolar macrophages are activated in many interstitial lung diseases, the present study was designed to evaluate a role for the alveolar macrophage as a source of the increased levels of fibronectin found in the lower respiratory tract in interstitial lung diseases and to determine if such fibronectin might contribute to the development of the fibrosis found in these disorders by being a chemoattractant for human lung fibroblasts. Production of fibronectin by human alveolar macrophages obtained by bronchoalveolar lavage and maintained in short-term culture in serum-free conditions was demonstrated; de novo synthesis was confirmed by the incorporation of [14C]proline. This fibronectin had a monomer molecular weight of 220,000 and was antigenically similar to plasma fibronectin. Macrophages from patients with idiopathic pulmonary fibrosis produced fibronectin at a rate 20 times higher than did normal macrophages; macrophages from patients with pulmonary sarcoidosis produced fibronectin at 10 times the normal rate. Macrophages from 6 of 10 patients with various other interstitial disorders produced fibronectin at rates greater than the rate of highest normal control. Human alveolar macrophage fibronectin was chemotactic for human lung fibroblasts, suggesting a functional role for this fibronectin in the derangement of the alveolar structures that is characteristic of these disorders.

Original languageEnglish
Pages (from-to)7147-7151
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number11
DOIs
Publication statusPublished - 1 Jan 1981
Externally publishedYes

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Interstitial Lung Diseases
Alveolar Macrophages
Fibronectins
Fibroblasts
Wounds and Injuries
Macrophages
Pulmonary Sarcoidosis
Lung
Idiopathic Pulmonary Fibrosis
Mononuclear Phagocyte System
Chemotactic Factors
Bronchoalveolar Lavage
Proline
Respiratory System
Fibrosis
Molecular Weight

ASJC Scopus subject areas

  • General

Cite this

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abstract = "Because cells of the mononuclear phagocyte system are known to produce fibronectin and because alveolar macrophages are activated in many interstitial lung diseases, the present study was designed to evaluate a role for the alveolar macrophage as a source of the increased levels of fibronectin found in the lower respiratory tract in interstitial lung diseases and to determine if such fibronectin might contribute to the development of the fibrosis found in these disorders by being a chemoattractant for human lung fibroblasts. Production of fibronectin by human alveolar macrophages obtained by bronchoalveolar lavage and maintained in short-term culture in serum-free conditions was demonstrated; de novo synthesis was confirmed by the incorporation of [14C]proline. This fibronectin had a monomer molecular weight of 220,000 and was antigenically similar to plasma fibronectin. Macrophages from patients with idiopathic pulmonary fibrosis produced fibronectin at a rate 20 times higher than did normal macrophages; macrophages from patients with pulmonary sarcoidosis produced fibronectin at 10 times the normal rate. Macrophages from 6 of 10 patients with various other interstitial disorders produced fibronectin at rates greater than the rate of highest normal control. Human alveolar macrophage fibronectin was chemotactic for human lung fibroblasts, suggesting a functional role for this fibronectin in the derangement of the alveolar structures that is characteristic of these disorders.",
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AU - Crystal, Ronald

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