Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways

Uli S. Herrmann, Tiziana Sonati, Jeppe Falsig, Regina R. Reimann, Paolo Dametto, Tracy O’Connor, Bei Li, Agnes Lau, Simone Hornemann, Silvia Sorce, Uli Wagner, Despina Sanoudou, Adriano Aguzzi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Prions induce lethal neurodegeneration and consist of PrPSc, an aggregated conformer of the cellular prion protein PrPC. Antibody-derived ligands to the globular domain of PrPC (collectively termed GDL) are also neurotoxic. Here we show that GDL and prion infections activate the same pathways. Firstly, both GDL and prion infection of cerebellar organotypic cultured slices (COCS) induced the production of reactive oxygen species (ROS). Accordingly, ROS scavenging, which counteracts GDL toxicity in vitro and in vivo, prolonged the lifespan of prion-infected mice and protected prion-infected COCS from neurodegeneration. Instead, neither glutamate receptor antagonists nor inhibitors of endoplasmic reticulum calcium channels abolished neurotoxicity in either model. Secondly, antibodies against the flexible tail (FT) of PrPC reduced neurotoxicity in both GDL-exposed and prion-infected COCS, suggesting that the FT executes toxicity in both paradigms. Thirdly, the PERK pathway of the unfolded protein response was activated in both models. Finally, 80% of transcriptionally downregulated genes overlapped between prion-infected and GDL-treated COCS. We conclude that GDL mimic the interaction of PrPSc with PrPC, thereby triggering the downstream events characteristic of prion infection.

Original languageEnglish
Article numbere1004662
JournalPLoS Pathogens
Volume11
Issue number2
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Prions
Anti-Idiotypic Antibodies
Infection
Tail
Reactive Oxygen Species
Unfolded Protein Response
Excitatory Amino Acid Antagonists
Antibodies
Calcium Channels
Endoplasmic Reticulum
Down-Regulation
Ligands

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Herrmann, U. S., Sonati, T., Falsig, J., Reimann, R. R., Dametto, P., O’Connor, T., ... Aguzzi, A. (2015). Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways. PLoS Pathogens, 11(2), [e1004662]. https://doi.org/10.1371/journal.ppat.1004662

Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways. / Herrmann, Uli S.; Sonati, Tiziana; Falsig, Jeppe; Reimann, Regina R.; Dametto, Paolo; O’Connor, Tracy; Li, Bei; Lau, Agnes; Hornemann, Simone; Sorce, Silvia; Wagner, Uli; Sanoudou, Despina; Aguzzi, Adriano.

In: PLoS Pathogens, Vol. 11, No. 2, e1004662, 2015.

Research output: Contribution to journalArticle

Herrmann, US, Sonati, T, Falsig, J, Reimann, RR, Dametto, P, O’Connor, T, Li, B, Lau, A, Hornemann, S, Sorce, S, Wagner, U, Sanoudou, D & Aguzzi, A 2015, 'Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways', PLoS Pathogens, vol. 11, no. 2, e1004662. https://doi.org/10.1371/journal.ppat.1004662
Herrmann US, Sonati T, Falsig J, Reimann RR, Dametto P, O’Connor T et al. Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways. PLoS Pathogens. 2015;11(2). e1004662. https://doi.org/10.1371/journal.ppat.1004662
Herrmann, Uli S. ; Sonati, Tiziana ; Falsig, Jeppe ; Reimann, Regina R. ; Dametto, Paolo ; O’Connor, Tracy ; Li, Bei ; Lau, Agnes ; Hornemann, Simone ; Sorce, Silvia ; Wagner, Uli ; Sanoudou, Despina ; Aguzzi, Adriano. / Prion Infections and Anti-PrP Antibodies Trigger Converging Neurotoxic Pathways. In: PLoS Pathogens. 2015 ; Vol. 11, No. 2.
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