Prime-boost vaccination strategy against avian influenza and Newcastle disease viruses reduces shedding of the challenge viruses

Nermeen M. Ismail, Ayman H. El-Deeb, Mohamed Emara, Hoda I. Tawfik, Nabil Abdel Wanis, Hussein A. Hussein

Research output: Contribution to journalArticle

Abstract

In the present study, we carried-out assessment of efficacy of different immunization strategies using two bivalent vaccine formulations containing antigens of inactivated Newcastle disease virus (NDV-genotype VIId) and reassortant highly pathogenic avian influenza virus (H5N1-HPAIV) mixed with Montanide ISA71 and Montanide Gel02 as adjuvants. The efficacy of the prepared vaccines was evaluated by determining the cellular and humoral immune responses. In addition, protection against H5N1-AIV and NDV-genotype VIId challenge viruses post vaccination was assessed when Montanide-Gel02 based vaccine was inoculated in 10-days-old specific pathogen free chicks intraocularly once, twice or once followed by a boost with the Montanide ISA71 based vaccine. The cytokines profile analysis demonstrated that the prime-boost strategy induced the highest up-regulation in interferon-gamma (11.39-fold change) and interleukin-6 (14.12-fold change) genes expression. Also, enhanced lymphocytes proliferation was recorded beside increased antibody titers with protection levels reaching 50 and 60% against H5N1 and NDV challenge; respectively. Immunization with Montanide ISA71 inactivated vaccine induced 80% protection; however, the prime-boost combination afforded complete protection (100%) in the challenged chickens against mortality, clinical signs and virus shedding. Finally, these results highlight the significance of considering not only different vaccine platforms but also vaccination strategies to maximize protection against AIV and NDV with regards to the longevity of the vaccine-induced immune response.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalVirusDisease
DOIs
Publication statusAccepted/In press - 26 Jun 2018

Fingerprint

Bird Diseases
Virus Shedding
Newcastle disease virus
Influenza in Birds
Vaccination
Vaccines
Viruses
Immunization
Genotype
Specific Pathogen-Free Organisms
Inactivated Vaccines
Humoral Immunity
Orthomyxoviridae
Cellular Immunity
Interferon-gamma
Chickens
Interleukin-6
Up-Regulation
Lymphocytes
Cytokines

Keywords

  • Genotype VIId
  • H5N1
  • Montanide
  • Newcastle disease virus
  • Prime-boost

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Prime-boost vaccination strategy against avian influenza and Newcastle disease viruses reduces shedding of the challenge viruses. / Ismail, Nermeen M.; El-Deeb, Ayman H.; Emara, Mohamed; Tawfik, Hoda I.; Wanis, Nabil Abdel; Hussein, Hussein A.

In: VirusDisease, 26.06.2018, p. 1-9.

Research output: Contribution to journalArticle

Ismail, Nermeen M. ; El-Deeb, Ayman H. ; Emara, Mohamed ; Tawfik, Hoda I. ; Wanis, Nabil Abdel ; Hussein, Hussein A. / Prime-boost vaccination strategy against avian influenza and Newcastle disease viruses reduces shedding of the challenge viruses. In: VirusDisease. 2018 ; pp. 1-9.
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