Preliminary crystallographic study of hemoglobin from buffalo (Bubalus bubalis)

A low oxygen affinity species

Balasubramanian Moovarkumudalvan, Ponnuraj Sathya Moorthy, Kamariah Neelagandan, Mondikalipudur Nanjappa Gounder Ponnuswamy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hemoglobin is a tetrameric, iron-containing metalloprotein, which plays a vital role in the transportation of oxygen from lungs to tissues and carbon dioxide back to lungs. Though good amount of work has already been done on hemoglobins, the scarcity of data on three dimensional structures pertaining to low oxygen affinity hemoglobins from mammalian species, motivated our group to work on this problem specifically. Herein, we report the preliminary crystallographic analysis of buffalo hemoglobin, which belongs to low oxygen affinity species. The buffalo blood was collected, purified by anion exchange chromatography and crystallized with PEG 3350 using 50mM phosphate buffer at pH 6.7 as a precipitant by hanging drop vapor diffusion method. Data collection was carried out using mar345dtb image plate detector system. Buffalo hemoglobin crystallizes in orthorhombic space group P21212 1 with one whole biological molecule (α2β2) in the asymmetric unit with cell dimensions a=63.064Å, b=74.677Å, c=l 10.224Å.

Original languageEnglish
Pages (from-to)213-215
Number of pages3
JournalProtein and Peptide Letters
Volume16
Issue number2
DOIs
Publication statusPublished - 1 Feb 2009
Externally publishedYes

Fingerprint

Buffaloes
Hemoglobins
Oxygen
Metalloproteins
Lung
Chromatography
Carbon Dioxide
Anions
Buffers
Blood
Iron
Phosphates
Vapors
Tissue
Detectors
Molecules
hemoglobin Buffalo

Keywords

  • Asymmetric unit
  • Bubalus bubalis
  • Crystallization
  • Hemoglobin
  • Low oxygen affinity
  • Orthorhombic

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology

Cite this

Preliminary crystallographic study of hemoglobin from buffalo (Bubalus bubalis) : A low oxygen affinity species. / Moovarkumudalvan, Balasubramanian; Moorthy, Ponnuraj Sathya; Neelagandan, Kamariah; Ponnuswamy, Mondikalipudur Nanjappa Gounder.

In: Protein and Peptide Letters, Vol. 16, No. 2, 01.02.2009, p. 213-215.

Research output: Contribution to journalArticle

Moovarkumudalvan, Balasubramanian ; Moorthy, Ponnuraj Sathya ; Neelagandan, Kamariah ; Ponnuswamy, Mondikalipudur Nanjappa Gounder. / Preliminary crystallographic study of hemoglobin from buffalo (Bubalus bubalis) : A low oxygen affinity species. In: Protein and Peptide Letters. 2009 ; Vol. 16, No. 2. pp. 213-215.
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