Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo

Svetlana Glushakova, Yanjie Yi, Jean-Charles B. Grivel, Anjali Singh, Dominique Schols, Erik De Clercq, Ronald G. Collman, Leonid Margolis

Research output: Contribution to journalArticle

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Abstract

Many HIV-1 isolates at the late stage of disease are capable of using both CXCR4 and CCR5 in transfected cell lines, and are thus termed dual- tropic. Here we asked whether these dual-tropic variants also use both coreceptors for productive infection in a natural human lymphoid tissue microenvironment, and whether use of a particular coreceptor is associated with viral cytopathicity. We used 3 cloned dual-tropic HIV-1 variants, 89.6 and its chimeras 89-v345.SF and 89-v345.FL, which use both CCR5 and CXCR4 in transfected cell lines. In human lymphoid tissue ex vivo, one variant preferentially used CCR5, another preferentially used CXCR4, and a third appeared to be a true dual-tropic variant. The 2 latter variants severely depleted CD4+ T cells, whereas cytopathicity of the virus that used CCR5 only in lymphoid tissue was mild and confined to CCR5+/CD4+ T cells. Thus, (a) HIV-1 coreceptor usage in vitro cannot be unconditionally extrapolated to natural microenvironment of human lymphoid tissue; (b) dual-tropic viruses are not homogeneous in their coreceptor usage in lymphoid tissue, but probably comprise a continuum between the 2 polar variants that use CXCR4 or CCR5 exclusively; and (c) cytopathicity toward the general CD4+ T cell population in lymphoid tissue is associated with the use of CXCR4.

Original languageEnglish
JournalJournal of Clinical Investigation
Volume104
Issue number5
Publication statusPublished - Sep 1999
Externally publishedYes

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Lymphoid Tissue
HIV-1
T-Lymphocytes
Viruses
Cell Line
Infection
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo. / Glushakova, Svetlana; Yi, Yanjie; Grivel, Jean-Charles B.; Singh, Anjali; Schols, Dominique; De Clercq, Erik; Collman, Ronald G.; Margolis, Leonid.

In: Journal of Clinical Investigation, Vol. 104, No. 5, 09.1999.

Research output: Contribution to journalArticle

Glushakova, S, Yi, Y, Grivel, J-CB, Singh, A, Schols, D, De Clercq, E, Collman, RG & Margolis, L 1999, 'Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo', Journal of Clinical Investigation, vol. 104, no. 5.
Glushakova, Svetlana ; Yi, Yanjie ; Grivel, Jean-Charles B. ; Singh, Anjali ; Schols, Dominique ; De Clercq, Erik ; Collman, Ronald G. ; Margolis, Leonid. / Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo. In: Journal of Clinical Investigation. 1999 ; Vol. 104, No. 5.
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