Power of family-based association designs to detect rare variants in large pedigrees using imputed genotypes

Mohamad Saad, Ellen M. Wijsman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently, the "Common Disease-Multiple Rare Variants" hypothesis has received much attention, especially with current availability of next-generation sequencing. Family-based designs are well suited for discovery of rare variants, with large and carefully selected pedigrees enriching for multiple copies of such variants. However, sequencing a large number of samples is still prohibitive. Here, we evaluate a cost-effective strategy (pseudosequencing) to detect association with rare variants in large pedigrees. This strategy consists of sequencing a small subset of subjects, genotyping the remaining sampled subjects on a set of sparse markers, and imputing the untyped markers in the remaining subjects conditional on the sequenced subjects and pedigree information. We used a recent pedigree imputation method (GIGI), which is able to efficiently handle large pedigrees and accurately impute rare variants. We used burden and kernel association tests, famWS and famSKAT, which both account for family relationships and heterogeneity of allelic effect for famSKAT only. We simulated pedigree sequence data and compared the power of association tests for pseudosequence data, a subset of sequence data used for imputation, and all subjects sequenced. We also compared, within the pseudosequence data, the power of association test using best-guess genotypes and allelic dosages. Our results show that the pseudosequencing strategy considerably improves the power to detect association with rare variants. They also show that the use of allelic dosages results in much higher power than use of best-guess genotypes in these family-based data. Moreover, famSKAT shows greater power than famWS in most of scenarios we considered.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalGenetic epidemiology
Volume38
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014

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Keywords

  • Burden test
  • Inheritance vectors
  • Kernel statistic
  • MCMC
  • Mixed linear model
  • Sequence data

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)

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