Potency analysis of cellular therapies

The emerging role of molecular assays

David F. Stroncek, Ping Jin, Ena Wang, Betsy Jett

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

Potency testing is an important part of the evaluation of cellular therapy products. Potency assays are quantitative measures of a product-specific biological activity that is linked to a relevant biological property and, ideally, a product's in vivo mechanism of action. Both in vivo and in vitro assays can be used for potency testing. Since there is often a limited period of time between the completion of production and the release from the laboratory for administration to the patient, in vitro assays such are flow cytometry, ELISA, and cytotoxicity are typically used. Better potency assays are needed to assess the complex and multiple functions of cellular therapy products, some of which are not well understood. Gene expression profiling using microarray technology has been widely and effectively used to assess changes of cells in response to stimuli and to classify cancers. Preliminary studies have shown that the expression of noncoding microRNA which play an important role in cellular development, differentiation, metabolism and signal transduction can distinguish different types of stem cells and leukocytes. Both gene and microRNA expression profiling have the potential to be important tools for testing the potency of cellular therapies. Potency testing, the complexities associated with potency testing of cellular therapies, and the potential role of gene and microRNA expression microarrays in potency testing of cellular therapies is discussed.

Original languageEnglish
Article number24
JournalJournal of Translational Medicine
Volume5
DOIs
Publication statusPublished - 30 May 2007
Externally publishedYes

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Assays
MicroRNAs
Testing
Gene Expression Profiling
Microarrays
Therapeutics
Genes
Signal transduction
Flow cytometry
Cytotoxicity
Bioactivity
Signal Transduction
Stem cells
Flow Cytometry
Metabolism
Gene expression
Leukocytes
Stem Cells
Enzyme-Linked Immunosorbent Assay
Technology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Potency analysis of cellular therapies : The emerging role of molecular assays. / Stroncek, David F.; Jin, Ping; Wang, Ena; Jett, Betsy.

In: Journal of Translational Medicine, Vol. 5, 24, 30.05.2007.

Research output: Contribution to journalReview article

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